The skin is the outer coverage of the human body and, at the same time, the largest organ that has important functions, such as preventing excessive water loss, preventing the introduction of microorganisms into the body and maintaining the proper temperature of the body. Skin is the first member of the body that is against the injuries. In the present age, one of the most important challenges is the repair of skin wounds. Wound dressing is one of the cheapest, most affordable and the best suited to wound healing. Nevertheless, extensive research is under way to achieve the ideal properties of existing dressings. The purpose of this study was to design a polyvinylalcohol-chitosan-poly (vinylpyrrolidone) / calcium peroxide Nanocomposite fiber membrane and characterization it. In order to, at the beginning Calcium peroxide nanoparticles were synthesized by chemical deposition method. Then, after optimization of poly (vinyl alcohol-chitosan-poly(vinylpyrrolidone) fiber membrane, poly-vinyl alcohol-chitosan-poly(vinylpyrrolidone) / calcium peroxide nanocomposite membrane was synthesized and characterized by electrospinning. The effect of increasing of different weight percentages of calcium peroxide nanoparticles (5,10 and 15 wt%) on morphological changes, mechanical and biological properties of fiber scaffolds and the realese of hydrogen peroxide from fiber membrane was assessed. The results showed that calcium peroxide nanoparticles with particle size of 25 ± 6.9 nm and spherical morphology were synthesized. The mean diameter of polyvinyl alcohol-chitosan-poly(vinylpyrrolidone) fiber membrane with 40-40-20 volume ratio of three components was 123 ± 6,2 nm, that has good mechanical properties (Youngs modules = 28.87±8 MPa , Strain percent = 10.7% and tensile strength = 2.9 ± 1.26 MPa), degradability (70 ± 2% after 14 days immersion in phosphate buffer solution) and water absorption (251.7% ) Was selected and used in the next step. The results showed that increasing 5% weight of calcium peroxide nanoparticles significantly increased the tensile strength of the fiber membrane (3.4 ± 2.3 MPa). All the membranes had hydrogen peroxide release at a controlled rate. Increasing calcium peroxide concentration leads to significant hydrogen peroxide release in solution. Due to the the results of fibroblastic cell culture, it was observed that increasing the amount of calcium peroxide to 15% weight led to reduction in cell viability of the samples due to its significant release of hydrogen peroxide. In conclusion the poly (vinyl alcohol-chitosan –poly(vinylpyrrolidone) / calcium peroxide Nanocomposite fiber membrane containing 5-10% calcium peroxide can be suitable membrane for wound healing. Key word: Wound healing, Calcium peroxide, Chitosan, Poly(vinyl Alcoho)l, Poly(vinyl Pyrrolidone), electrospinning.
