Recovery of low grade sulphide ores particullary mine tailings attained much attention by increasing demande and price of basic metals including Copper, Cobalt and Nickel in recent decades. Among Hydrometallurgical processes, Bioleaching was considered as an efficient process due to some advantages such as low capital cost, Eco-friendly and more selectivity. This research aimed to study the effect of chemical characteristics of water including pH, culture medium type, microorganism type and sodium chloride concentration as well as pulp density on the recovery of copper, cobalt and nickel in the presence of mixed culture contained Iron and Sulphur oxidizing microorganisms from sulphide tailing of Golgohar, sirjan. In this research mesophile and moderate thermophile microorganisms were used at 35 ?C and 45?C, respectively. In order to understand the process mechnisms and the effect of parameters, the effect of mentioned critical factors on the electrochemical behavior of sulphide tailing was studied under conditions close to bioleaching medium. Bioleaching experiments were conducted in pH=1.2, 1.5 and 1.8, pulp density 5% and 10% (w/v), Norris and 9K culture medium, Sodium Chloride concentration of 0, 5 and 10 gr/l and in the presence of local water and obtained water from flotation circuit of pyrite. The bioleaching results indicated that maximum relative recovery (90.5%)(the weighted average of Copper, Cobalt and Nickel versus price) was achieved in pH=1.8, Sodium Chloride concentration of 10 gr/l, pulp density 10%, Norris culture medium and in the presence of moderate thermophile microorganisms so that Copper, Cobalt and Nickel recoveries were obtained 66.2%, 87.5% and 87%, respectively. On the other hand maximum Iron concentration in the solution was 9880 ppm which was obtained under coditions of Norris culture medium, Sodium Chloride concentration of 5 gr/l, pH=1.5 and in the presence of moderate thermophile microoganisms. The results presented that increasing pH from 1 to 1.5 increased metal recoveries while increasing pH from 1.5 to 2.5 decreased it. Also, increasing Sodium chloride concentration increased the dissolution rate which is reduced after 10 days because of Jarosite sediment formationon the mineral surface. Metal recovery increased from 83.3% to 85.5% in the presence of local water whilst in the presence of flotation circuit water, it decreased from 83.3% to 55.5% which is maybe attributed to depressant effects of foltaion reagents. The results also demonstrated that increasing in pulp density from 5% (W/V) to 10% decreased normalized recovery from 87.9% to 65%which is resulted due to increasing in the shear force. Also it was found that culture medium type has not significant effect on the metal recovery, However with regard to economical factors (less concentration), it is proposed to use Norris culture medium