The global consumption of fibers has increased along with the population growth and general increase of the living standards. In 2011, the worldwide fiber consumption exceeded 85 million tonnes, which consists of around 40 and 60% cellulosic and man-made fibers, respectively. This huge amount of fibers is processed into apparel, home textiles, and industrial products, ending up as waste after a certain time delay. Even though current management of waste textile mainly comprises incineration and landfilling, the waste textiles have a potential to serve as an alternative feedstock for production of biological products via microbial fermentation. Bioethanol is one of the renewable fuels which can be produced from waste textiles by a process containing pretreatment, hydrolysis, fermentation, and purification steps. In this study, different chemical pretreatments by alkali solvents (NaOH, NaOH/urea, NaOH/thiourea and NaOH/urea/thiourea), concentrated phosphoric acid and organic solvent N-methyl-morpholine-N-oxid were evaluated for production of ethanol from textile. The alkaline pretreatment was performed at four different temperatures of -20, 0, 23, and 100 ?C for 1 h, where as the phosphoric acid and NMMO pretreatments were conducted at 50 ?C for 1h and at 120 ?C for 3h, respectively. Pretreatment of textile with both phosphoric acid and NMMO resulted in separation of the cellulosic fibers from the polyester. However, cellulose and polyester fibers remained together in the alkaline pretreatment, and they were separated in the enzymatic hydrolysis instead where the cellulose was converted into soluble glucose. Treated and untreated textiles was subjected to enzymatic hydrolysis at 45 ?C for 72 h using 30 FPU cellulose and 60 IU ?-glucosidase per grams of substrates. Furthermore, the hydrolysates were subjected to simultaneous saccharification and fermentations (SSF) at 36 ?C for 72 h using 15 FPU and 30 IU per grams of substrates. Results of hydrolysis showed that glucose yield was increased by pretreatments from 46.3% for untreated textile to more than 80, 99, and 94% for alkali and phosphoric acid and NMMO-treated textiles, respectively. In addition, ethanol yield was improved from 36.1% for untreated textile to more than 70% for alkali and phosphoric acid treated samples. Considering the weight and composition of materials before and after of the process, cotton was separated from polyester fibers with percentage of 84.9 and 95.4% for phosphoric acid and NMMO, respectively. In addition, through enzymatic hydrolysis of alkali pretreated textile more than 90% of cellulose was separated, while only 51. 8% of cellulose was separated by enzymatic hydrolysis of untreated textiles. Considering the characterization of the polyester, the crystallinity was increased by treatments, while viscosity was decreased . Key Words ioethanol, cotton, enzymatic hydrolysis, polyester, textile.