: Pulsed packed column is one of the important equipment that is applied for liquid–liquid extraction, because of its high efficiency and low required space, in nuclear, pharmaceutical, petrochemical and food industries. In this project, experiments were done on a pulsed packed column pilot plant. In pulsed extraction columns energy is supplied via input mechanical pulses. Input of pulses, act as a mechanicalmixer and also produce dropswithsmallerdiameters, which in turnincreasethe column efficiency. Prominent features of pulsed extraction columns are as follows:high efficiency in extraction, simple design, stable and reliable operation, no moving parts and convenient operation with radioactive and corrosive solutions. Considered variables in experiments include: flow rates of dispersed and continuous phases and frequency of pulses. In this project, leach liquor solution from "Bandar Abbas yellow cake manufactury" was used. In many uranium mines such as Gachin in Bandar Abbas, sulfuric acid is used for leaching operation on ore after crushing operation. This leach liquor containing uranium, is extracted by specific extractant solvent, like trioctyleamine(TOA). This operation, named Amex, have been used for many years, but for the first time, in the current project this process was carried out with pulsed packed column. Experiments were performed in different operational conditions including different frequency of pulses and different flow rates of dispersed and continuous phases.According to the previous studies, the optimum condition is volume percent of 4 for trioctyle amine in kerosene (as diluent) solution, pH=1 and Q c /Q d =1. In this optimum case 98% of extraction could be reached. In the current project extraction of uranium using pulsed packed column in the above optimum condition was investigated. After each experiment and reaching steady state, aqueous phase was sampled for analysis to measure Uranium extraction coefficient, using valves No.1, 4 ,7 on the active part of the column and the waste. The photography from three different section of column was done tomeasure the d 32 using a digital camera. Diameter of droplets decrease from bottom to the top of column because of impact to packed and mass transfer from continuous phase to dispersed phase. This led to increase the hold up. In extraction process, leach liquor containing uranium is the continuous phase of pulse packed column and trioctyle amine solution is the dispersed phase of column. Also, studies on the effect of operating variables on the flooding and holdup of a pulsed packed column are considered. Holdup is one of the most important variables in the design of a pulsed extraction column. The holdup is needed to calculate the interfacial area per unit volume and the absolute phase velocities. Keywords: Liquid-Liquid Extraction , Pulsed Packed Column, Holdup, Leach liquor, Uranium, TOA