Recently electrospinning technique ha attractedgreatattentio a the simplest and least expensive mea iproducing polymer nanofiber with diameter ranging fromnano to micro scale. Alginate (Alg) i aabundant,nontoxic, nonimmunogenic, and biodegradable polymer derived from marine brow algae, one of themajor components of ECMs in human tissue. Since Alg is a hydrophilic polymer, Alg-based material facilitate uniform cell seeding and cell ingrowth into the matrix. Alg is also known to breakdown to simpler glucose type residue which i completely bioabsorbable . Alg has been studied extensively owing to its unique tissue compatibility and applicatio i tissue engineering, including the regeneration of skin, Cartilage, bone, liver, and cardiac tissue. Poly (vinyl alcohol)(PVA) is a semi crystalline polymer that is biocompatible and water-soluble, and it has a good formation ability for fibrou materials. It flexibility and toughne are good, so it is a typical synthetic polymer that is used to improve the physical propertie through mixing with other material that have poor physical properties. PVA with functional grou i useful i practical investigatio of functional polymer because of its easy preparatio as a bulk material, films, and fibers. Gelatin (Gel) is a natural biodegradable polymer derived from collagens that contains many functional groups (glycine, proline, glutamic acid, hydroxyproline, arginine, alanine, aspartic acid, and other amino acids). Owning to its excellent biocompatibility and biodegradability properties, Gel has been widely used and studied in regards to many biomedical applications,including wound or burn dressings; surgical treatments; tissue engineering of bone,skin, and cartilage.The aim of this thesis was to fabricate PVA-Gel-Alg nanofiber via electrospinning method. First, the spinnability of PVA solutio wa observed. Traarent solutions of PVA were prepared by dissolving PVA in DW at about 80°C, with continuous stirring for 2–3 h. I regard to the formatio of continuou and uniform nanofibers observed by optical microscopy photographs, the solutions with concentrations of 15%, applied voltage of 13.5 kV, flow rate of 0.6 mL/hr and TCD of 13.5 cm were found to be spinnable and produce smooth and uniform nanofiber. These process parameter were used for all next exprimens. Pure Alg aqueous solution can’t be electrospun. No continues jet were formed at equal or less than 2 wt.% of Pure Alg solution polymer concentration while above this concentration the solution viscosity is too high for electrospinning process. Good morphology and beadfree nanofiber were obtain by electrospinning of PVA/ Alg with 16.5 wt.% total polymer concentration (15 wt% PVA and 1.5 wt% Alg). Regarding Alg solution will precipitate under pH = 3.5, Acetic acid were use to make blended solutio with different pH, particularly 3,4 and 5. To made PVA/Gel solution at these pH, precise amount of Gel was added to 15 wt% PVA to made PVA/Gel solution at 1, 3 and 5 wt% Gel concentration. PVA/Gel could successfully be electrospun at 3 wt% of Gel on pH = 3. Then this solution was added to PVA/Alg solution at 70:30, 50:50 and 30:70 volume ratio to made PVA-Gel-Alg electrospinning solution and it observed that the best morphology and bead free nanofiber could be obtain from the solution with volume ratio of 30:70 (PVA/Alg : PVA/Gel ). In