Liquid membrane separation techniques perform the separation of the solute from the solvent in a single stage by combining the extraction and stripping of the solute. Low energy requirements, feasibility of handling very low concentrations of the solute, and high flux of mass transfer have made liquid membrane separation processes more attractive and economical over other separation processes. Among various types of liquid membranes, emulsion liquid membrane (ELM) has the advantages of having high surface areas, high rate of extraction/stripping process and high efficiency. ELM technique is established as an effective separation method for separation and enrichment when the species to be extracted is present in very low concentration. ELM technique have been successfully applied for separation and enrichment of traces of heavy metals, precious metals, proteins, and enzymes. Selection of a suitable carrier, emulsifier, emulsion size, and dilute phase among other operational conditions are the key factors that control the ion extraction efficiency. Glycine is a sweet-tasting, white crystalline solid. Among the 20 natural amino acids that are the building blocks of proteins, Glycine is the simplest natural amino acid, is not optically active, and has a unique non-chiral structure. In living systems, Glycine is used to help create muscle tissue and convert glucose into energy. This amino acid is necessary to maintain the central nervous and digestive systems healthy. Glycine has been shown to promote the gastric absorption of certain drugs, including aspirin.. Glycine can be synthesized by biosynthesis or from protein hydrolysis, however, its separation and purification from fermentation broth or from protein hydrolysates is a rather difficult task. The purpose of this research was to study the separation of Glycine from synthetic aqueous solution using ELM technioque. Batch extraction of Glycine using emulsion liquid membrane process was carried out. Effects of operating parameters such as surfactant concentration, carrir concentration, volume ratio of emulsions to aqueous external phase, stirring speed and emulsification speed on rate of extraction were examined. Central composite response surface methodology (CCRSM) was applied to optimize the extraction of Glycine from an aqueous solution. The membrane phase consists of D2EHPA (di-2-ethylhexyl phosphoric acid), Span 80, and liquid paraffin. The size distribution of droplets in the emulsion was characterized by Dynamic light scattering (DLS). Results showed that the mean droplet size distribution of water in oil emulsion droplets was about 84 nm. A small aggregation of droplets with an average size of more than 800 (?m) was found. This is because of the instability of the emulsion and coalescence of the droplets during the process. Under optimum conditions, results showed that about 90.5% extraction could be achieved. The optimal values for surfactant concentration, carrier concentration, treat ratio, stirring speed and emulsification speed were 4.94%v/v, 0.54 (wt/v), 0.38 (v/v), 621(rpm), and 18500 (rpm), respectively. The membrane swelling is an undesirable phenomenon that may occur in all ELM operations. Swelling during Glycine extraction was caused by water traort and membrane rupture. Results showed that membrane swelling was increased as surfactant concentration, carrier concentration, and agitation speed increased. Keywords Emulsion liquid membrane, Glycine, Extraction, swelling.