Copper, nickel and cobalt metals are such valuable elements in industrial tailing resources which separated from copper, nickel, cobalt and iron low grade solutions by hydrometallurgical routes. These strategic metals have several applications in different industries. The aim of this research is to separate and recover by product valuable metals from low grade tailing solutions by bio hydrometallurgical process and nickel latterite resources. The results of chemical characterization investigations indicated that copper, cobalt and nickel are the most promising metals that that have the ability to further processing studies. At first, the investigations and analysis of copper separation process by solvent extraction route were conducted using Chemorex CP-150 oxime base extractant. Therefore at first step, to evaluate the appropriate contact time of aqueous and organic phases, some kinetic experiments were performed at time intervals of 0.5, 2,4,8,10,12 and 16 min. The results of kinetic studies indicated that the suitable contact time for copper extraction experiments was 2 min. In the next step, extraction experiments were done based on a full factorial 2 3 full factorial design of experiments. The purpose of these experiments is to determine optimum levels of parameters on copper extraction while co-extraction of other metal ions is the lowest. The results of these experiments showed that best conditions of copper extraction using Chemorex CP-150 as an organic solvent were pH of 2.3, A:O ratio of 1:1 and low concentration levels of solution 0.5 g/L copper, 0.25 g/L nickel, 0.125 g/L and 0.1 g/L iron. In this regard, in order to further investigation of copper, solvent extraction capability and evaluate effective parameters such as extractant concentration, solution pH, phase ratio, initial solution concentration on optimum copper extraction and also extraction isotherms containing copper counter- current extraction using solvent extraction method , further experiments were done. It is interfered that under conditions of 10% (v/v) extractant, 0.6 g/L solution, A:O ratio of 2:1, 2 min contact time, two extraction stages were sufficient to achieve %99.9 copper extraction. According to obtained conditions, last experiments were performed on the real bioleach solutions. Due to the presence of metal impurities especially iron in bio hydrometallurgical process, iron removal was done using ammoniojarosite process before copper extraction. After that the copper solvent extraction and cobalt, nickel separation were conducted using hydroxide and sulfide precipitation processes. The results of precipitation indicated that under conditions pH of (8.5-9), sulfide to metal molar ratio of 1.8, temperature of 50°C and 30 min detention time, %96.13 nickel and %92.04 cobalt were removed. Also low grade latterite resources were examined. After leaching process, the nickel recovery was obtained %98.78 and about %99.62 nickel and %99.43 cobalt were removed by sulfide precipitation under the conditions pH of (8.5-9), sulfide to metal molar ratio of 1.8, temperature of 50°C and 30 min detention time. It is suggested that investigation synergism effect of other extractants on copper extraction, cobalt, nickel separation using organic extractants namely Cyanex 272 and versatic acid, and also air assisted solvent extraction process can be investigated.