Colloidal gas aphrons are stable micro bubbles with a multi-layered shell that is generated by severely stirring an aqueous surfactant solution. The high interfacial area introduced by the small size of aphron bubble and high stability of colloidal gas aphrons due to the presence of a thin soapy shell surrounding the core of the bubbles, make them interesting in various processes such as protein recovery. The purpose of this research is enrichment of superoxide dismutase from affordable and available chicken liver. Aphron was generated by severely stirring polysorbate-20 solution using a homogenizer. One of the most important properties by which the quality of aphrons is assessed is stability. Surfactant concentration, homogenizer speed, stirring time and fraction of the distance of homogenizer’s disperser from the surface of the solution are chosen as operational variables affecting the quality of aphron generation. Optimization is performed based on the stability of aphron. For experimental design, data modeling and optimization of the operational parameters, minitab17 software and response surface methodology is used. Optimal values of surfactant concentration, homogenizer speed, stirring time, and fraction of distance of the homogenizer’s disperser from surface of the solution are determined as 7/5 mM, 24000 rpm, 9 min and a quarter of total solution’s height from surface of the solution, respectively. Under optimal operational conditions, aphron was generated and used for protein recovery. Surfactant concentration, pH and volume ratio of aphron to enzyme solution were chosen as operational variables affecting the enrichment ratio. Optimal values of surfactant concentration, pH and volume ratio of aphron to enzyme solution were obtained as 10mM, 4 and 1/8, respectively. Activity of SOD was measured. Electrophoresis results indicated that the activity of the enzyme is preserved. Key words : Colloidal Gas Aphron, Stability, Superoxide dismutase enrichment, Response Surface Methodology.