The Nanostructures fabricated from biomolecules are attracting increasing attention owing to their biocompatibility, their ability for specific molecular recognition , simple chemical and biological modification and easy availability for bottom-up fabrication. Many biomolecules such as peptides can interact and self-assemble into highly ordered supramolecular architectures. In among the block structures based on peptides, peptide Di-phenylalanine (FF) and its derivatives are very simple, which can be made to different nanostructures with new optical mechanic and electrochemical properties. In Among the applications of this dipeptide, using it in the form of drug nanocarriers like hydrogels that has good biocompatibility and high biodegradability and suitable drug delivery. A simple method of synthesis of the hydrogels is solving monomer dipeptide (Di-phenylalanine) in hexa-fluoride-propanol solvent and diluting in other solvents such as chloroform and toluene . In this study, nano-carriers that are like to peptide hydrogels structures , used for loading flufenamic acid drug (FFA) , which is an Anti-inflammatory drug. To evaluate the effect of solvents on the formation of hydrogels, 28 samples of the solvents ethanol, methanol, toluene, acetone, xylene and dimethyl sulfoxide with 10, 25, 40, 70 and 100 ratios was prepared. In order to study the morphology transmission in self-assembly of the dipeptide(FF) system , scanning electron microscope (FESEM) was used. Also, In order to investigate the intermolecular interactions in the dipeptide (Di-phenylalanine) gel and in the microcrystal state, Fourier transform Infrared Spectra analysis (FTIR) was done and to evaluate the molecular self-assembly in different hydrogel samples , X-ray diffraction analysis (XRD) was used. Then, according to the results of analysis, sample of hydrogel containing 25% ethanol and 75% toluene was selected for loading flufenamic acid drug at concentrations of 25, 50, 75 and 100 micrograms per cc. Thus, different concentrations of drug was loading into the sample and using the spectrophotometer analysis, the optimum concentration of the load drug was determined. The optimum concentration (25 micrograms per ml) was used for loading into the 4 samples hydrogel which are (25% methanol and 75% toluene); (30% ethanol and 70% Xylene); (30% ethanol and 70% acetone) and ( 25% acetone and 75% toluene). After taking four samples, the absorption of suspension was read by using spectrophotometer and found that Drug with the optimum concentration is loaded into the hydrogels compeletly.By using the results and calculations found that each 5 samples do not show release of drug from hydrogel over time an hour. The results indicate that the hydrogel samples (75% toluene and 25% methanol) and (70% acetone and 30% ethanol) after 2.5 hours and the hydrogel samples (75% toluene and 25% ethanol), (75% toluene and 25% acetone) and (70% xylene and 30% ethanol) after three hours release completely from the hydrogel. To evaluate the kinetics of the release drug from hydrogel ,the Kursymer and coworkwer relation was used to determine the n and it was found that all samples are contain n ??greater than 0.5 and have non-Fick mechanism. It can be concluded that the mechanism of release are two diffusion and erosion type, however, in these samples erosion mechanism that include both chemical reaction and mass transfer phenomena was overcome on diffusion mechanism. Key Word Hydrogel structure, Peptides , Self-assembly, Di-phenylalanine, Mechanism of drug release.