anti-microbial activity. However mechanically inferior feature of Chitosan in the wet state has limited its usage. On the basis of above mentioned characteristics, it can be seen that Chitosan and PHB have mutually complementary potentials. Therefore it is reasonable to expect that their individual deficiencies would be overcome if PHB and Chitosan could be well blended together. But some difficulties have been frequently encountered about blending PHB with Chitosan due to tow main problems: (1) melting processing technique cannot be applied since chitosan has high melting point and PHB will start to decompose before melting Chitosan; (2) there are very few common solvents available for Chitosan and PHB. In the present work, PHB/Chitosan blend scaffolds where prepared using trifluoro acetic acid as a new co-solvent, combined with salt-leaching technique. Fabricating PHB/Chitosan blend scaffolds according to a Taguchi experimental design, the material parameters as the PHB concentration, Chitosan and salt content were investigated. The scaffolds were analyzed by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR), contact angle, water absorption and degradation measurements. FT-IR band intensity of the carbonyl stretching absorption from PHB revealed that the crystallization of PHB in these blends is suppressed when the concentration of Chitosan was increase. SEM images showed a thin and rough top layer with a nodular structure, supported with a porous sub layer in blend scaffolds. According to SEM images, there were two kinds of pore in the blend scaffold: cubic pores that arise from salt-leaching and spherical small pores that arise from immersion precipitation. The contact angle of blend scaffolds where influenced by salt content and chitosan concentration; with increasing the salt content, the top layer became rougher and the contact angle increased, but with increasing the Chitosan concentration the contact angle decreased. As well as water absorption measurements showed that the water absorption of blend scaffolds increase with increasing the Chitosan concentration.degradation of blend scaffolds in phosphate buffer solution () has been studied by measuring weight of samples and measurement of pH for 14 weeks. In vitro degradability investigation indicated that the degradation rate of blend scaffold was higher than pure PHB scaffolds, and the dissolution of chitosan could neutralize the acidity of PHB degradation products. Maximum degradation rate has been seen for prepared scaffold with 90wt% NaCl and 40wt% Chitosan. The Obtained results suggest these newly developed PHB/Chitosan blend scaffolds may serve as a three-dimensional substrate in cartilage tissue engineering.