Nowadays, due to limited oil resources and also increasing concerns about protecting the environment, much attention has been drawn to replacing packaging films based on synthetic polymers with environmental friendly and biodegradable films. On the other hand, regarding the large costs of extracting oil, producing raw materials, film production and finally disposing the waste products of these films, it is essential to find a suitable alternative for these films which should cause less pollution and be more cost effective. In this study to answer the need for replacing synthetic polymer based packaging films, biodegradable and environmental friendly nanocomposites consisting of three parts based on thermoplastic starch, reinforced with cellulose nanofibers and graphene oxide nanoplatelets were produced and investigated. Results showed that by simultaneously using cellulose nanofibers and graphene oxide nanoplatelets, mechanical, thermal and gas barrier properties of thermoplastic starch and thermoplasticstarch/cellulose nanofibers films were significantly improved. For example the gas barrier properties of the samples consisting of 3% graphene oxide nanoplatelets were higher than commonly used polymers such as PET, PVC and PE. XRD patterns showed that nanoplatelets were successfully exfoliated and dispersed inside the matrix. Also the morphology of the samples were changed due to incorporation of graphene oxide nanolplatelets. Keywords: Packaging, Biodegradable nanocomposite, CNF, TPS, GO; Nowadays, due to limited oil resources and also increasing concerns about protecting the environment, much attention has been drawn to replacing packaging films based on synthetic polymers with environmental friendly and biodegradable films. On the other hand, regarding the large costs of extracting oil, producing raw materials, film production and finally disposing the waste products of these films, it is essential to find a suitable alternative for these films which should cause less pollution and be more cost effective. In this study to answer the need for replacing synthetic polymer based packaging films, biodegradable and environmental friendly nanocomposites consisting of three parts based on thermoplastic starch, reinforced with cellulose nanofibers and graphene oxide nanoplatelets were produced and investigated. Results showed that by simultaneously using cellulose nanofibers and graphene oxide nanoplatelets, mechanical, thermal and gas barrier properties of thermoplastic starch and thermoplasticstarch/cellulose nanofibers films were significantly improved. For example the gas barrier properties of the samples consisting of 3% graphene oxide nanoplatelets were higher than commonly used polymers such as PET, PVC and PE. XRD patterns showed that nanoplatelets were successfully exfoliated and dispersed inside the matrix. Also the morphology of the samples were changed due to incorporation of graphene oxide nanolplatelets. Keywords: Packaging, Biodegradable nanocomposite, CNF, TPS, GO;