The skin is a heterogeneous viscoelastic substance whose properties depend on the age, the person and the location of the skin. Various diseases or environmental injuries such as accidents, surgeries, chemical and chronic burns can easily destroy skin tissue. Application of nanofiber mats made by electrospining method due to high surface area, morphological control and other properties, in medicine and tissue engineering science is the most important tool for researchers in restoring damaged tissues. Poly (?-caprolactone) (PCL) is a non-toxic synthetic polyesters with suitable physical properties, and semi-crystalline. Gelatin (Gel) is a natural hydrophilic polymer synthesized from amino acids linked by peptide bonds. Cellulose nanofibers (CNF) are one of the most important natural crystalline polymers with low density, high mechanical properties, and favorable economic value. In this research, scaffolds of these three biodegradable and biocompatible polymers with a concentration of 10% (w/w) PCL/Gel polymer in AA-FA (3: 1) solvents with different ratios (70:30) P70, (50:50) P50, and (30: 70) P30 and different percentages of cellulose nanofibers of 1, 2, 3, and 6% were prepared and electrically spun under optimized conditions. To optimize the solution conditions, viscosity and electrical conductivity of different polymer concentrations were measured. Fourier transform spectroscopy was carried out to confirm the presence of all three polymer materials. To investigate the quality of elctrospun mats, morphology and porosity of nanofibers mats were examined via SEM. In addition, the mechanical properties and crystallinity of the electrospun scaffolds were studied using Zwick machine and XRD, respectively. Moreover, the hydrophilic properties of the scaffolds were investigated through the contact angle measurement. The rate of degradation of polymeric scaffolds immersed in solution at 37 ?C to 60 days and also the morphological studies of scaffolds and ultimately Fibroblast cell culture and endemic sclera Osmith (MTT) on scaffolds were studied. Among the most important results: reaching a 95.98 nanometer diameter for nanofibers in the P70 compound, the mean pore size of all nanowire polymeric scaffolds ranging from 1.6 to 6.5 nm, increasing the hydrophilic properties after addition CNF, increasing mechanical properties and maintaining the stability of nanoparticulate polymer scaffolding with P70 polymer ratio after adding CNF to 2% by weight and achieving a tensile stress of 3.24 MPa and a tensile modulus of 25.75 MPa, the highest crystallinity of 56.82% after Adding CNF and enhancing the adhesion and growth of fibroblast cells following the addition of CNF to polymeric PCL/Gel scaffolds. Keywords: Tissue engineering, Poly (?-caprolactone), Gelatin (Gel), Cellulose nanofiber (CNF), Fibroblast cell.