The excessive use of fossil fuels and the release of various pollutants has caused many environmental problems. Due to the limited availability of these resources, the renewable and reliable and environmentally friendly sources are needed. Among existing biofuels, biodiesel and bioethanol have been widely considered by the lack of high environmental pollutants. Biohydrogen is also known as a high-energy clean fuel. Various renewable sources have been proposed for the production of these biofuels in recent years. Among renewable sources of third generation energy, micro-algae, has attracted much attention. Green microalga Chlorella sorokiniana was used in this study due to its ability to grow in different conditions and producing a significant percentage of lipid and carbohydrates. Different culture conditions were applied for cultivation of Chlorella sorokiniana to investigate the productivity of lipid and biomass. Photoautotrophic culture was performed in two ways: carbon dioxide was obtained by shaking and aeration, and receiving bicarbonate at concentrations of 1 to 10 g/l of sodium bicarbonate. The best culture condition considering the culture time was obtained at the concentration of 4 g/l of sodium bicarbonate. The heterotrophic culture was tested using glucose at 8 concentrations ranging from 2 to 30 g/l. photoheterotrophic culture was performed in the mentioned concentrations of glucose with 12 hours of lihgting and 12 hours of darkness throughout the day. The potential of hydrogen production was investigated during this cultivation. At the concentration of 2 g/l glucose, the highest amount of biomass was produced and at higher concentrations, a decreasing trend in biomass was observed. In mixotrophic culture glucose and sodium bicarbonate mixtures in constant concentration of 2 grams per liter of sodium bicarbonate with 5 different glucose concentrations between 2 and 10 g/l was used. The best growth conditions were observed in the concentration of 4 g/l glucose. The best cultivation was selected based on the amount of biomass production and growth time to produce biodiesel, bioethanol and biohydrogen. In the study, photoheterotroph culture with 2 g/l glucose, 37 ° C, pH 6.8 and exposure period of 12 hours of brightness and 12 hours of darkness chose as optimum culture. The amount of biomass production in these conditions was 0.995 g per liter of culture medium. The maximum growth rate was calculated to be 0.69 per day. In order to increase the amount of lipid, nitrogen shock was applied to the culture medium, which is increased the lipid content from 6% to 11%. Due to the decrease of biomass amount through nitrogen shock up to 0.331 g/l, the productivity of lipid under this shock was reduced. Glucose consumption in the concentration of 2 g/l was 69%, which is the highest amount of glucose consumption in comparison with higher concentrations of glucose. The hydrogen produced under these conditions was measured at 389 milliliters per liter of culture medium. After acidic hydrolysis with 3% sulfuric acid on the biomass whose lipid was extracted, 0.053 grams ethanol per gram of biomass, was produced by Saccharomyces serevisiae in 12 hours. Keywords: 1- Photoautotrophic, Photohetrotrophic Mixotrophic cultivation 2- Biodiesel 3- Biohydrogen 4- Chlorella sorokiniana