In this study investigate the potential of bio hydrogen and acetone-butanol-ethanol (ABE) production as a clean fuel of cellulosic substrate. Also, to increase the production efficiency, the co-culture of clostridium acetobutylicum and enterobacter aerogenes were used. Closteridium acetobutylicum is an obligate species and small amount of oxygen can completely inhibit its production metabolism, therefor the presence of an oxygen reducing agent such as cysteine is necessary. In this study, an anaerobic strain Enterobacter aerogenes , which is capable of consuming oxygen in the medium can also act as a reducing agent in the culture medium. In the first step, initially, using glucose as a substrate, the effect of the primary pH on the production process was studied in the constant glucose concentration. Then, in the optimum pH of hydrogen production, the effect of primary glucose concentration on the production process was investigated. The results of fermentation in a constant glucose concentration of 20 g/L showed that pH= 7 with 4575 ml of hydrogen per liter of medium and 3.1 g/l of acetone-butanol-ethanol as the optimum pH of hydrogen production and pH= 6 with Hydrogen production 1762.5 ml/L and ABE 6.7 g/L were obtained as the optimum pH of the ABE production. Also, in the study on the effect of initial concentration of glucose on the production efficiency with a fixed pH of 6.8, the concentration of 40 g/l with 5400 ml/L of biohydrogen production and 11.09 g/l of ABE was the optimal concentration of hydrogen production and ABE due to higher efficiency more known. Also, based on the results obtained from the optimal pH value of the production of Biohydrogen and ABE, pure culture and co-culture were used to compare the production at two biohydrogen and ABE initial pH. The results revealed that the yield of the products in the optimal pH of hydrogen production in the co-culture was 3.16 and 1.6 times more than pure culture of enterobacter and clostridium respectively, and at optimal pH of the solvent production, the co-culture 79% and 1.5% higher solvent production of pure enterobacter and clostridium , respectively. The other benefit of co-culture is the lack of use of a reducing agent. Finally, solid waste paper, paper pulp, newspaper and pure cellulose were used as the primary cellulose feed in the fermentation process. The primary feeds of cellulose were converted into sugars by cellulase enzyme. In order to improve the activity of the enzymes, they were pretreated by alkaline pre-treatment at low temperature the crystalline structure distrupt and enzyme hydrolysis increased with 54%, 10%, 72.3% and 12% respectively for cellulose, pulp, paper and solid waste of the paper mill. Productivity yield per gram of solid substrate for pre-treated cellulose 0.263gABE/gSubstrate and 99.5 mlH2/gSubstrate, pre-treated pulp 0/294gABE/gSubstrate and 142 mlH2/gSubstrate, pre-treated newspaper 0.137gABE / gSubstrate and 66.3 mlH2/gSubstrate and paper mill solid waste 0.012 g ABE/gSubstrate and 11 mlH2/gSubstrate were obtained. Also according to the final results, it can be concluded that the pretreatment process on paper mill solid waste feed is an additional step Also, the results of the FTIR test confirm this claim.