Halo archaea bacteria are bacteria that need high concentrations of salt to grow. These bacteria are usually isolated from salt lakes and require a concentration of about 0.6 M sodium chloride to 5 M sodium chloride to grow. Halofrax Mediterranei is a bacterium that can consume a wide range of carbon sources such as sucrose, maltose, etc. It can also grow at concentrations of 170 to 200 g / l sodium chloride. This bacterium grows aerobically and produces intracellular pigment. The major pigment in Halo archaea bacteria is ?-bacteriorubrin. In this study, Halofrax Mediterranei were cultured to produce bacteriorubrin in culture medium prepared with reverse osmosis concentrate. Thus, the ability to produce carotenoids using reverse osmosis concentrate was investigated. reverse osmosis concentrate is a brine from reverse osmosis systems that contains high amounts of BOD, COD and salt ions that can cause harmful damage to the environment. The nutrients needed for bacterial growth, including salts, a nitrogen source, and a carbon source — the carbon source in this study was glucose — were added to the reverse osmosis concentrate. Culture media with concentrations of 5, 10, 15, 20 and 25 g / l glucose were prepared and batch culture was continued for 7 days. On the last day, the parameters of biomass absorbance at 600 nm, residual glucose concentration in culture medium (g / l), pH and bacteriorubrin absorbance at 495 nm were measured. The two categories of culture medium were the main culture medium (using distilled water) and the culture medium prepared by reverse osmosis excretion effluent, respectively. It was shown that the culture medium prepared with reverse osmosis rejection effluent had optimal and better results and also the optimal concentration of glucose required for bacterial growth and carotenoid production according to the obtained data, 2% (20 g / l). In the culture medium prepared with reverse osmosis concentrate. On average, after 7 days, the amount of biomass absorbance at 600 nm at a concentration of 2% glucose for the main culture medium and culture medium prepared with reverse osmosis concentrate, It was 2.79 and 3.43 respectively which in comparison with concentrations less than 2% of glucose, the average biomass absorbance was 1.5 and 1.95, respectively. Therefore, the optimal glucose concentration was 2%. Also, the absorbance of bacteriorubrin at 495 nm in the main culture medium and the culture medium prepared by reverse osmosis concentrate with 2% glucose concentration was 0.635 and 0.782, respectively. Continuous culture was also performed to obtain specific growth rate and bacterial productivity rate. The specific bacterial rate, ?MAX was 0.27 1/d. Continuous culture was performed in a volume of 2 liters with two growth rates ? of 0.15 D / 0.1 and 0.2 1/d, which yielded 0.76 g / Ld and 1.014 g / Ld, respectively. Continuous culture was also scaled in a 70-liter pilot. Key words: bacterioruberin, Haloferax meditarranei, reverse osmosis concentrate