The plasma membrane that surrounds all living cells must be strong enough to prevent the permeation of unwanted ions and molecules,but also flexible enough.Solutions of amphiphilic molecules such as lipids in water are characterized by a wide range of length scales. These molecules usually resemble semiflexible rods with a length of the order of 1–2 nm, which is already large compared to the atomic size .0.1 nm.We perfect to use Dissipative Particle Dynamic method to simulate biological membranes. In this method, several atoms are united into a single simulation particle. Forces in DPD are pairwise additive, conserve momentum, have no hard core, and are short-ranged.Our bilayer membranes are composed of surfactants which consist of hydrophilic head groups and hydorophobic tail groups.Double-chain surfactants have symmetric chains or maybe asymmetric chains. In addition, we incorporate the bending stiffness of these chains. Unsaturated carbon bonds can change the stiffness of a membrane lipid. We model the stiffness of the chain by introducing this bond bending potential.Using DPD simulations, we observe the self-assembly of the surfactant molecules into bilayer membranes. We also calculate the surface tension of the bilayers. The stress profile is gained and is similar to that found in coarse-grained Molecular Dynamics simulations, but requires a fraction of the computational cost.The effect of changes in the chain length and stiffness of the surfactants on the properties of the model membranes are studied. We observe that changes of the stiffness have significant effects if these changes are made close to the head group of the surfactant. If, on the other hand, changes are made at the end of the tail of the surfactant, the properties of the bilayer are similar to the properties of a bilayer consisting of flexible chains. We observed as the tail length increases, the area per surfactant increases then we compare these results with the theoretical calculations of Cantor on a lattice model. We were able to give to our surfactants a special chemistry structure and do this membrane simulation more actual. It becomes our work quite distinct from most of studies. Another goal in this work is to calculate the diffusion coefficient of the water through the modeled membrane. Then we compare this coefficient with theoretical results and other simulations like Molecular Dynamics simulation and in this way we could validate our results. Dissipative Particle Dynamics therefore allows the study of the equilibrium behavior of fluid bilayer membranes hundreds of times larger than that can be achieved using Molecular Dynamics simulations, and opens the way to the investigation of complex mesoscopic cellular phenomena. Key words : Dissipative particle dynamicsc, Biological Membrane