In the first study, an attempt was made to synthesize heterocyclic compounds of 1,6-diamino-3,5-di-cyano-2-oxopyridine. For this purpose, three catalysts with a rich surface of secondary and tertiary amines were prepared. First, bulk g-C3N4 was prepared from urea pyrolysis, and then g-C3N4 nanosheets were prepared from thermal oxidation of bulk g-C3N4. The IS@SM catalyst was prepared, including ionic salts containing piperazine grafted to silica-coated magnetic nanoparticles' surface. To prepare IS@SM catalyst, at first silica-coated magnetic nanoparticles (SM) were prepared by sol-gel method. Then, piperazine was attached to the silica surface by 3-chloropropyl triethoxy silane as a linker agent. All three catalysts were evaluated for four-component reaction, and IS@SM was selected for higher efficiency and shorter reaction time. In the following, the reaction conditions were optimized in different solvents under ultrasonic irradiation. Also, the product yield of the reaction exposed to ultrasound irradiations was compared with reflux conditions, in which ultrasonic waves had higher efficiencies and shorter reaction times. Under optimal conditions, several derivatives were synthesized using various aldehydes and ketones. Among the synthesized products, aromatic aldehydes were more efficient than aliphatics. In the second part of this study, the synthesis of benzothiazolopyridine compounds, i.e., 1-amino-2- (benzo [d] thiazol-2-yl) -3-aryl-3H-benzo [4, 5] thiazolo [2-a, 3] pyridine-4-carbonitrile was studied under ultrasonic irradiation and conventional thermal method using piperidine in EtOH. To evaluate the effect of ultrasound technique, some derivatives were synthesized using some aldehydes under ultrasound irradiation and reflux method. The ultrasonic irradiation afforded several privileges such as shorter reaction time, cleaner reactions, and high yields. Ultrasound technique reduced the time of reactions from 150-480 min to 80-200 min and improved the efficiency from 10-71 to 30-89. Also, reactions with aldehydes, including electron-releasing groups, lead to low yield in a long time, or the final product's formation was not possible. In the next section, to evaluate the anti-cancer properties of the synthesized derivatives, from each series, four compounds with phenyl, 4-nitrophenyl, 4-chlorophenyl, and 4-methoxyphenyl substitution were selected, and several cell lines were considered for each group. For the first group, MCF-7 breast cancer and HeLa cancer cell line were selected, and for the second group, two breast cancer, MCF-7, MDA-Mb, and a normal breast cell line (MCF-10A) were selected. The interactions between these compounds and some of the most important protein receptors in these cells were simulated using molecular docking. Bonding interactions related to the first group compounds with this receptor were favorable in terms of energy and unfavorable for the second group. Finally, these compounds were treated on the preferred cell lines by MTT assay. The results obtained from cell culture were in good agreement with the results obtained from the cloning, and the first group compounds showed a stopping property, while the second group compounds, in most cases, did not have such a property.