Glucose is a high energy density, non-toxic, and easy to handle and store fuel. Also, it is available in nature, therefore direct glucose fuel cell is a desirable candidate for the fuel cell investigations. In the recent years, a large number of researches have been carried out and reported on improving the performance of nanocatalysts for both anode and cathode in direct glucose fuel cell. In this project, anode electrodes were made by Hypermec TM Pt free nano catalysts and its performance in glucose electrooxidation reaction compared to conventional palladium anode electrode. Glucose concentration effect on the performance of nano structural anode electrodes was investigated and optimum concentration of glucose was determined, which was 0.1M of glucose and 1M of KOH. Morphological characterization and performance of Hypermec TM Pt free and palladium base electrode were studied by scanning electron microscopy (SEM) and electrochemical methods such a Cyclic Voltammetry (CV), Chronoamperometry, current-voltage curves and Electrochemical impedance spectroscopy(EIS). Study of poisoning, durability and stability of catalysts investigated in this thesis. According to the results, Pd/C10%wt nanocatalyst had a more stability compared to Hypermec TM Pt free nanocatalyst. Finally, a membrane electrode assembly (MEA) with Pt free nano structure electrode and anion exchange membrane were fabricated and developed. The mentioned MEA employed in direct glucose fuel cell. Maximum power density of 22mw.cm -2 were yielded for the MEA containing Hypermec TM Pt free porous anode electrode and 13 mW.cm -2 for the MEA containing Pd/C10%wt porous electrode at the ambient fuel and oxidant pressure. the obtained results are comparable to other works results. Charge transfer and mass transfer resistances were studied by both of electrodes in fuel cell. According to the results, Hypermec TM Pt free electrode had faster electron and mass transfer rates compared to Pd/C10%wt electrode .