(I) Two phosphorus ylide Ag and Ni complexes driving from mono-phosphonium ligand were synthesized and characterized using various techniques. Montmorillonite was used as a solide support for incorporating the cationic part of complexes to product heterogeneous catalysts. Low amount of catalysts were used for the hydrogen transfer reactions of aromatic nitro compounds at room temperature. (II) A silver (I) complex of ? -keto phosphorus ylide driving from reaction of silver trifluoromethanesulfonate (AgOTf) and mono-phosphonium ligand was synthesized and characterized using various techniques X-ray crystallography was used to determine the crystal structure that show an ylide C bound to a soft metal center was displayed for the Ag complex. The data by the analysis revealed that the Ag complex could bind to DNA through the groove binding mode. The emission titration of bovine serum albumin (BSA) with the complex showed a static process for the fluorescence quenching mechanism of BSA. In addition, the donor (BSA) - acceptor (Ag complex) distance was calculated by using fluorescence resonance energy transfer (FRET). The results of competitive binding by means of Warfarin, Ibuprofen and Digoxin site markers revealed that the complex was bound to the site I of BSA. It was shown that the complex had remarkable in vitro cytotoxicity against melanoma (B16F0) and colon carcinoma (C26) cancer cell lines. Finally, molecular docking studies were used for the determination of DNA and BSA-Ag (I) complex binding. (III) The ylide-phosphonium salt [PPh 3 CH 2 C(O)CH 2 Cl] + Cl - was reacted with Pd(OAc) 2 to give the chloro-bridged dinuclear complex [Pd{C(H)PPh 3 C(O)CH 2 Cl}(?-Cl)(OAc)] 2 , which experienced bridge cleavage reactions with triphenylphosphine (PPh 3 ), pyridine (Py) and sodium azide (NaN 3 ) to prepare the new orthometallated complexes. The complexes were identified and characterized using various techniques. X-ray crystallography was used to determine the crystal structure of one of the complexes. The DNA and BSA binding properties of two prepared complexes were checked out using fluorescence spectroscopy, electronic absorption and molecular docking studies. The complex-CT-DNA interaction for the complexes via groove binding with the partial intercalation, according to the results. The quenching of BSA emission through the complex was of the static form, as shown by the reactivity toward BSA. Site marker competitive analysis showed that the two Pd complexes could bind to the BSA molecule in one site. Cytotoxic activity of the Pd complexes against K562 and Jurkat cell lines demonstrated the potent effect against K562 cancer cell lines. Cell cycle analysis showed the effect of compounds on cell cycle arrest by decreasing cell DNA content and inducing cell death. The binding distances with BSA based on the Foster’s theory illustrated that the energy transfer could occur from BSA to the ylide complexes. Finally, molecular docking studies also verified the interaction mode of the complex with DNA and BSA.