In this project, Palladium complexes containing metformin and benzylamine derivatives have been synthesized. (1) [Pd(Met) 2 ](OAc) 2 (2) [Pd(DL-metylbenzylamine)(Met)](NO 3 ) (3) [Pd(N,N-dimethylbenzylamine)(Met)](NO 3 ) (4) [Pd(N-Ethylbenzylamine)(Met)](NO 3 ) (5) [Pd(N-tertiobutylbenzylamine)(Met)](NO 3 ) Every five complexes were characterized on the basis of elemental analyses, FT-IR and NMR (1H, 13C) spectroscopy. In the second part of the project, biological activity of the metformin and complex (1) and (2) was evaluated by examining their ability to bind to CT-DNA using UV-Vis, fluorescence, thermal denaturation and circular dichroism spectroscopy, and according to the experiments, performed, partial intercalation interaction was suggested for metformin and groove interaction was suggested for complex (1) and (2). In this section, the interaction between metformin and complex (1) and (2) with BSA was studied. As a result of absorption UV-spectrums of protein in the presence and absence of different concentrations of metformin and complex (1) and (2), it was observed that reduction to higher wavelengths indicates the interaction of the complexes with BSA and the alterations in the intensity of the absorption band of BSA revealed that the interactions between BSA and metformin, complex (1) and (2) causes changes in BSA microenvironments around the amino-asids. While investigating the interaction between metformin and complex (1) and (2) with protein using fluorescence spectroscopy, the value of quenching rate constant (K q ), suggested statistical mechanism and the value of quenching rate constant of stern-volmer (K sv ) for metformin and both complexes, indicated the proper binding of BSA with metformin and complex (1) and (2). Competitive studies of sitemarkers of Warfarine, Ibuprofen and Digoxin were also performed to identify the binding site of the complex to BSA, and as a result of experiments, metformin has been placed in binding site II and both complexes have been placed in binding site I. In the last section of the thesis, the anti-tumor effects on the cell-domains two types of cancer cells, including MCF7, A549 and Hela was carried out and reported.