In this work the nano-sized nickel particles applied in nanocomposit have been synthesized by a bottom-up approach in two different temperature. Electrochemicalsensor for glucose was fabricated in an alkaline environment that contained a glassy carbon electrode modified with nano particle nickel by gamma- Aminopropyltriethoxysilane (KH550),(Ni/KH550) nanocomposite. Different methods such as fourier transform infrared (FT-IR), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), x?ray powder diffraction (XRD) were used to characterize the new nanocomposite Ni/KH550. The electrochemical measurements showed that the oxidation peak of glucose was appeared at a potential of about at 0.53 V (vs. Ag/AgCl) at a surface of the modi?ed glassy carbon electrode (GCE) in the cyclic voltammetry. Differential pulse voltammetry exhibited two wide linear dynamic ranges of 0.5–20 and 100–500 µmol /L glucose with a detection limit of 0.043 µmoL/l. Finally in the analysis of real samples we measured glucose in plasma and achieved results similar with data obtained by a commercially available glucometer.