In this thesis, palladium complexes [trans-Pd(C15H10NOCH3)2]Cl2 and [cis- Pd(C15H10NOCH3)(PPh3)2Cl]Cl were prepared using alkoxyme ligand. Then they were studied by different spectroscopic methods including: infrared spectroscopy and NMR. Then, their interactions with large biomolecules of deoxyribonucleic acid and bovine serum albumin were studied using various spectroscopic methods including ultraviolet absorption and fluorescence emission spectroscopy. The result was the confirmation of the binding of the prepared complexes to the deoxyribonucleic acid grooves and different binding sites of bovine serum albumin. Fluorescence emission data showed that the mechanism of extinction of albumin emission by these compounds is static. Competitive titration was performed on albumin with eosin-Y, ibuprofen and degoxin as site markers I, II and III. The antitumor activity and toxicity of these compounds were evaluated on cancer cell lines A549 (leukemia) and K562 by in vitro cytotoxicity test. The IC50 value showed the good activity of these complexes in inhibiting cancer cells