In this work, for the first time, full evaporation technique (FE) was combined with single-drop microextraction for determining low molecular weight alcohols (i.e., methanol, ethanol, and isopropanol) in biological fluids. In this technique, a 80-?L volume of sample was totally evaporated inside of a sealed glass vial at 60 °C. Then, the evaporated analytes were extracted into a 2-?L ethylene glycol droplet hanging from the tip of a microsyringe. The important factors effective on the extraction performance of the technique including extraction and evaporation temperatures, sample and solvent volumes, and extraction time were studied. The relative standard deviation of the method was lower than 6.7%. A good linearity (r 2 0.998) in the range of 0.03-7.00, 0.03-10.0 and 0.03-10.0 mg L -1 , was found for methanol, ethanol and isopropanol, respectively. The limit of detection and quantification of the method were 0.01 and 0.03 mg L -1 , respectively. The method was successfully applied for the determination of the analytes in urine, plasma and saliva samples. The analyte recovery for the real samples was in the range of 82-100%.