Films, Sponges, nanofibers and micro fibers is so interesting for researchers because of having low price , Abundance of raw materials , biocompatibility , biodegradability and possibility of make physical and chemical changes. In spite of plent iful researches in direction of Fiber production from regenerated silk fibroin via wet spinning methods or dry spinning, this fibers have limitations in physical and mechanical subjects in comparison with silk natural fibers. Having high price, environment al problems and Toxicity , Weak mechanical properties, brittleness and inflexibility are some of limitations of regenerated silk fibers. This research has done with purpose of using from waste of silk fibers in order to regeneration of fibroin and improving its process for producing regenerated fibroin fibers. In the following, study of improvement production process via wet spinning method in order achievement Properties required as Active ingredient carrier . Some of important effective factors that related to dissolution and regeneration of silk fibroin fibers Was evaluated . The settings of product process and improving different factors related to materials like type of solvent, dissolution conditions, and coagulation conditions has done in order to impro ving of silk fibroin fiber regeneration. In this research, Optimal conditions for fibroin fibers achievement with available facilities Was obtained. Appearance , surface and morphological properties of produced fibers was evaluated by using of light microsc ope, polarized light microscope , scanning electron microscope , FTIR, nd XRD Methods . Also for nontoxicity assessment of regenerated fibers, for regenerative medicine applications, MTT test and human fibroblast cell growth, Was evaluated on some of produced fibers. Results show the possibility producing fibers with controlled cross sectional surface, via changing the production conditions such as temperature, fibroin solution concentration and coagulation bath conditions. Among the attainment of this resear ch is achievement to Technical knowledge of silk fibroin fiber regeneration without need of dialysis pr o sses. Also silk fibroin fiber regeneration , via a simple, safe and economical method, and ability of fiber properties modification During the production proses is other advantage s of this research. Fibroin fibers containing the active ingredient ? - cyclodextrin were extracted and some of its properties, including noncytotoxicity and biocompatibility, were evaluated. The results indicate noncytotoxicity and very good biocompatibility properties of wet spun fibers.