In this research, nanofibers containing CipHCl drug are electrospun from biodegradable polymer in 3 steps for the purpose of healing of deep burns. The first step was the reparation of olutions of PVAc :PVC with mass ratios of 0 :100, 25 :75, 50 :50, 75 :25, 100 :0 in aqueous acetic acid solvents of 40%, 50%, 60% and subsequently were electrospun. In order to produce optimum nanofibers, the concentration of each component was determined. In the second step, 0.3 %( V/V) of sulfuric acid was added to all the solvents for improving the quality of electrospinning and nanofibers. The results showed that the electrical conductivity was increased by increasing sulfuric acid and the solutions viscosity slightly were decreased, while the average nanofiber diameters was decreased significantly, i.e. in blend with mass ratio of 25 :75, the average nanofiber diameter was decreased from 503.7 to 80 nm. In the third step electrospinning was carried out by adding the crystal powder of drug to the polymer solutions, which led to the improvement of nanofiber structure end quality. By making such a fine nanofibers, the degree of swelling was increased in such a way that for PVA nanowebs, in 24 hr, the degree of swelling was 2092%, for nanofibers with diameter of 180 nm, this enhancement was an increase of 150%. For analysis of the physical structure and probable chemical interference of drug and polymer, DSC analysis and for assessing the effect of electrospinning on physical structure of polymer and drug, and the distributing of drug in polymer chains, XRD was used. FTIR was applied to assure that there has been no interference between drug/polymers, polymer/polymer, and polymer/solvents. UV spectrophotometer was used to evaluate the amount of drug release from nanofiber non woven fibers, in which the phosphate buffer with PH, 6.8 was applied. The drug release results illustrated that the type of polymer, polymer ratios, the amount of drug, nanofiber diameter and thickness of nanofiber layer influence significantly on release profile, burst release, and rate and total time of drug release, in such a way that PVA nanofiber web containing 5% drug, had the maximum drug release time and the minimum burst release. The burst release was controlled by increasing the nanofiber diameter and nanofiber web thickness, separately. Totally, this study illustrated that by changing the parameters such as type of polymer, polymer ratios, amount of drug, web thickness and nanofiber diameter, different formulations will be obtained leading to various profiles for CipHCl drug release. Healing of the deeper burn, the formulation with higher ratio of PVA, due to the higher swelling, is preferred