In this study, production of stained antibacterial nylon 66 nanofiber was investigated by using antibacterial dye. Our experiments in this study can be summarized in two steps, in which the first step includes two parts. The first and the second steps involved Trying to synthesize antibacterial reactive dye from binding antibacterial material Proflavin ( shown with the letter P ) to reactive basis of dye ( shown with the letter D ) to produce nylon 66 nanofibers that contains stained antibacterial materials and then, investigation of their properties, respectively. In the first ste we tried to bind the dye reactive blue 4 to Proflavin and to bind the Proflavin to cyanuric chloride ( shown with the letter C ). In the first part, Reactive Blue 4 was purified by salting-out crystallization and solvent-non-solvent mixtures. All sample undergone FTIR spectroscopy and Thin Layer Chromatography(TLC) after purification. Then, all attempts focused on connecting Proflavin to Reactive blue 4 (Synthesis D+P) by 12 series of experiments. Again after these experiments, the resulting stained material from the reaction (D+P dye) undergone spectroscopy FTIR, UV-VIS and TLC.
