The chicken feathers is a very inconvenient and troublesome waste product of the poultry-farming industry. Feather ?bers have a relatively low density of approximately 0.8 g/cm3 and have good thermal and acoustical insulating properties. The keratin feather ?ber has many is being used by a considerable number of industries in applications such as short ?ber reinforcement for polymers. These fibers are also used for the production of a range of products in non-woven, ?lters and paper industries. Moreover, they are also a source of fillings in products like pillows. However, the amount of feather fibers used in the above mentioned industries is only a fraction of the whole feather production. Therefore in order to make a good use of this waste which is rich in protein, the recovery of the keratin from feather is presently the object of comprehensive investigations in many research centers. The keratinous feather from the poultry industries can be reduced and then solubilized to nearly pure keratin and then used in applications such as cosmetics or ?lm production (in a compressed form), biodegradable coatings, cell culturing substrates. In the powder form, keratin has also found applications in food, cosmetics and drug delivery applications. Generally speaking, powder can be produced through a number of techniques such as milling (ball, air) and spray drying. One of the drawbacks of these techniques is the rather wide distribution of the particles in the powder as well as a considerably higher particle size. Also the particles have not a unique shape. Electrospraying is a direct method for preparing solid particles which can be conducted under mild conditions. The literature review showed no report on the production of keratin nanopowder through electrospraying technique. So, in the present study, the production of keratin nanopowder through electrospraying will be persued. At first, feathe keratins was recovered from an aqueous solution of chicken feathers in urea, sodium dodecyl sulfate (SDS) and 2-mercaptoethanol. This keratin solution was dialyzed in order to get the solution containing only keratin macromolecules. The keratin in the solution was chemically modified with iodoacetic acid (IAA) to safeguard it against oxidation of thiol (-SH) Groups. This automatically enhances its solubility in organic solvent. The modified solution is dialyzed again to obtain the solution of modifies keratin. Then, freeze draying was employed to obtain the keratin cake. To prepare the electoapraying solution, the solubility as well as the electrospraying ability of the solution of keratin in a number of solvents was tested. The solvent tested were acetic acid, formic acid, hexafluoroisopropanol (HFIP), trifluoroacetic acid (TFA), ethanol, methanol, dimethylformamide and dimethylformamide. It was found out that only the solution of keratin in trifluoroacetic acid was suitable for electrospraying. In addition, the effect of some parameters in electrospraying, namely, concentration of keratin solution, voltage, feed rate, and needle–collector distance, on average particle size of keratin nanopowder was studied. The scanning electron micrographs showed that electrospraying is able to produce uniform spherical shape and size keratin nanopowder with average particle size as low as 60 nm. Also it was ascertained that lower concentrations, lower feed rates, longer needle-collector distances and increasing voltage up to18 kV lead to a decrease in the average particle size of keratin nanopowder. Higher voltages (more than 18kv) increased the average particle size. FT-IR and XRD studies showed that the structure of electrosprayed keratin is similar to raw keratin, but with a lower crystallinity index.