The purpose of the present research was to estimate the concentration of dyes i colored solution using a digital camera. To do so, 120 colored solutions were prepared by the combination of 1, 2, 3 or 4 dyes using six cationic dyes in different concentration. Then colors and transmission of the solutions were measured spectrophotometrically. Also, the colors and reflectance of the colored solutions were reproduced and reconstructed, respectively using the digital camera. The digital camera was characterized in order to obtain the optimized transfer matrix of RGB to CIEXYZ by a regression technique in which a third order polynomial was applied. Afterward the calculated CIEXYZs were used in order to reconstruct the transmission spectra of the solutions by the application of PCA, matrix R and matrix S methods. To improve the performance of the methods a yellow filter was placed in front of the digital camera's lens system. The filter increase the number of the camera's channels from 3 to 6. Finally, by the application of the reconstructed transmission spectra and the beer- lambert's law, the concentration of each dyes in the colored solutions were estimated. The results show that for each individual colored solution the accuracy of the reconstructed transmission spectra and consequently the accuracy of the estimation of the dyes are dependent to the accuracy of the digital camera characterization. However, the results of the criteria for the examination of the reconstruction methods and consequently the mean value of the criterion for the dye concentration, showed insignificant difference and therefore, it was concluded that all of the reconstructed methods showed similar performance.