Today, due to the widespread use of hydrophobic polymers such as polysulfone (PSf) in membrane industry, membrane modification using environmental friendly techniques such as plasma is highly encouraged. Additionally, the need for novel environmental friendly nanoparticles () with outstanding properties such as high population of surface hydrophilic functional groups, large specific surface area, facile synthesis, low cost and availability is highly acknowledged. Basil seed gum (BSG) an anionic plant-derived polysaccharide and surface-active hydrocolloid sparked the facile preparation of organic BSG with mean diameter of ~42 nm using planetary ball mill. Different analytical techniques including FESEM, TEM, FTIR, DLS, TGA, BET, zeta potential measurement and hydrophilicity quantification were considered to fully characterize the prepared BSG . PSf ultrafiltration (UF) membranes with different BSG contents, i.e. 0, 0.5, 1, 3 and 5% with respect to PSf weight, were prepared via the well-known nonsolvent-induced phase separation technique using PSf/ Polyvinyl pyrrolidone (PVP)/Dimethylformamide (DMF) 20/5/75 wt.% as casting dope. In fact, PVP was used as a hydrophilic polymer to increase solvent/nonsolvent exchange rate and enhance macrovoid formation. It was demonstrated that BSG content is of profound importance to produce PSf UF membranes with superior properties. In this regard, optimum BSG content was regarded as low as 0.5% by which pure water flux (PWF) as high as 299.8 L/(m 2 .h) with an increase by ~88% with respect to the pristine PSf UF membrane was achieved without compromising bovine serum albumin (BSA) rejection (~94%). Reduced surface roughness and preserved high thermal stability of PSf UF membrane was also observed using BSG . Interestingly, using 0.5% BSG , membrane fouling was highly mitigated as demonstrated by flux recovery ratio (FRR) of ~100% which remained approximately constant after five cycles ended at 325 min during evaluation of membrane anti-fouling properties. Ar/O 2 vacuum plasma treatment, a cost-effective and environmental friendly process, was also performed on PSf UF membrane containing 0.5% BSG and led to further enhancement of pure water permeability (PWP) by ~61% from 59.9 L/(m 2 .h.bar) to 155.32 L/(m 2 .h.bar) while FRR was preserved at ~100%. Overall, using 0.5% BSG and Ar/O 2 vacuum plasma treatment, an anti-fouling UF membrane with PWF as high as 310.6 L/(m 2 .h) under transmembrane pressure of 2 bar was prepared. The potential of PSf UF membranes containing BSG to purify methylene blue (MB) wastewater was also investigated. To further investigate the contribution of BSG to morphology and performance of PSf membranes, PVP was excluded from the casting dope and PSf concentration was increased to 30 wt.%. Membranes with different BSG contents, i.e. 0, 1, 3, 5, 10 wt.% with respect to PSf weight, were prepared via both vapor-induced phase separation (VIPS) and immersion precipitation (IP) techniques. It was shown that in contrast to IP, VIPS leads to macrovoid-free membranes regardless of BSG content. Additionally, membranes prepared via VIPS exhibited superior PWF and inferior MB rejection compared to those prepared via IP. Irrespective of membrane preparation technique, i.e. VIPS or IP, the highest PWF and MB rejection of ~99% at pH=12 were achieved at 1 wt.% and 5 wt.% BSG contents, respectively.