Nowadays, with the advancement of sciences in the field of textiles design and manufacture, various kinds of fabrics, fibers or yarns are used in combination; So goods with especial applications is going to be achieved. Manufacturing multilayer composites are typically used in various applications including filtration, protective sports clothing. With the advent different types of pollution that are the result of the progress and development of industries in the today world, there is a need to generate new protective textiles. When it comes to protection in clothing. In addition to protection against risk, it should also be enough comfortable. Paying attention to the physical properties of produced protective clothing is irrefutable. Among all methods of protection, the using of made membranes of nano fibers is one of the best options. It’s because of high resistance of flowing gas with optimal transmission of vapor and preventing the passage of particles with high molecular weight and comfortable functionalization. In this study, in the first phase, the substrates for producing membrane and methods of connecting in laminated textile were considered. To determine the optimal conditions for electro nylon 66 polymers, influence of production method parameters, type of substrate, concentration, speed of drum and time of electrospinning in physical properties of laminated textiles were investigated like air permeability, permittivity of water vapor, heat and moisture transfer, experiments tensile, shear and bending. Taguchi methods were used for optimizing cost, number of experiments and also systematic analysis of results. Influence of different parameters in experiments and significant of the parameters and also act of different levels of each parameters in different