Over the last several decades, an increase in longevity and life expectancy has raised the average age of the world’s population. This worldwide increase in the average age of the population has, in turn, led to a rapidly increasing number of surgical procedures involving prosthesis implantation. This has resulted in an urgent need for improved biomaterials and processing technologies for implants, more so for orthopaedic and dental applications. The gallbladder is an organ that sits just beneath the right lobe of the liver. Bile, which is a fluid produced by the liver and aids the digestion of food is released into the small intestine through the common bile duct. Biliary system disorders affect millions of people each year. A variety of diseases (tumor, cancer, inflammation and obstruction) can affect the bile ducts. Reconstruction of the common bile duct is one of the most common challenges for hepatobiliary surgeons to repair bile duct injury or defect. In this study, after reviewing the steps taken for selection of methods and production processes based on research and past experience, samples of prosthetic bile ducts were produced by polyurethane electrospinning. These samples were produced under 0.205 and 0.17 ml per hour feed rate, 4.7 and 4.1 kV applied voltage and 40 and 60 RPM rotational speed. After that variety of tests undertaken on the prosthesis including morphological properties, mechanical properties, degradation and water absorption properties, was performed and compliance of the prosthesis was determined and the results of the tests were analyzed. In order to achieve optimum sample, the results obtained with the corresponding properties of human bile ducts were compared and the best sample made ??of polyurethane was chosen. In order to improve surface properties, a thin layer polytetrafluoroethylene polymer was covered on the inner surface of the prosthesis by electrospinning method. Also, by analysis of results of morphological properties, a good sample of polytetrafluoroethylene was obtained and by FTIR the optimum conditions for the removal of the fiberizing polymer, was determined to be 400 ° C for 3 minutes. Finally, first for the inner layer, electrospinning of polytetrafluoroethylene was performed and heated. For the exterior layer and the main body of the prosthesis, polyurethane was covered o polytetrafluoroethylene heated sample with 0.205 ml per hour feed rate, applied voltage of 4.7 kV and 60 RPM roller rotation speed electrospinning condition and the final prosthesis sample was obtained. The compliance property of the sample was very close to the natural organ in the human body. Keywords: Common bile duct; Electrospinning; Polyurethane nanofiber; Polytetrafluoroethylene nanofiber;