Surgical site infection after surgery can be a serious complication that may sometimes cause to death. The role of sutures in surgical site infection is an argument that has been the focus of attention. This issue leads to the production of absorbable surgical sutures with antibacterial properties. In some cases, the production of suture from a specific polymer or antibacterial agent for the particular application is considered and since the investigation on the industrial scale production of such yarns is expensive, so electrospinning method is suitable for the production of yarn with these characteristics. The purpose of this study was to investigate the properties of nano-fiber yarn produced via two nozzles Electrospun with a mixture of two polymers poly glycolic acid, poly lactic glycolic acid and nano-silver antibacterial agent. In the first part, in order to manufacture nano yarn, factors affecting the electrospinning such as concentration, voltage, feed rate, distance between two nozzles and the distance from the nozzles to collector were optimized. In the next section, after the optimized conditions, with using optimal concentration of polymer, different three concentrations of antibacterial agent were considered. Then, the antibacterial test was conducted with four different microbes and finally the best sample of anti-bacterial properties was determined. Eventually, the mechanical properties such as strength without knot and knotted yarn (square and surgery knot) were analyzed. The results of this test showed that the samples with each other have not statistically significant differences. In the next section, the biodegradability nano-fibrous suture yarns were examined, within seven days Results showed that the mechanical properties and weight of yarns quickly are reduced. No capillarity is desired in any case of suture application due to the spread of microorganism with the body fluid i betwee the fiber i sutures. In next approach, capillary rise in the nano-fibrous yarn and braid vicryl suture were studied. Comparison of the capillary rise demonstrated that the yarn produced by electrospinning has less capillary property. Finally, the fracture morphology of nano-fibrous yarn has evaluated. The results of SEM images showed fracture morphology of yarn is very similar to stake-and-socket mechanism. Also, analyze of fracture morphology of single nanofibers were similar to mushroom ends. Keywords : mechanical and physical properties, nano-fibrous suture, antibacterial agent, nano silver particles, PLGA, PGA