Cartilage is a connective tissue that has little capabilities in restoration due to poor blood circulation and slow metabolism. Tissue engineering is an appropriate method for cartilage tissue regeneration due to its suitable morphological, physical, chemical, mechanical, and biological properties. Studies on electrospun gelatin/chitosan (CS/GEL) scaffolds have shown that even though this scaffold has its biological properties and good cellular behavior, it does not have the desired mechanical properties to be used in cartilage tissue engineering. Therefore, the present study examines the effect of single-walled carbon nanotubes functionalized by carboxyl groups ( HOOC-sTNWS( on CS/GEL scaffolds to improve the biological, physical, and mechanical properties of the scaffold the present study. The polymer solution was prepared by mixing the ratio of 29/29 (w/w) of chitosan solution 97(w/v) with gelatin solution 297(w/v), which are both solved in a solvent system of trifluoroacetic acid(TFA)/dichloromethane(DCM) with a ratio of 19/99 (v/v). the final concentration of the solution was 79%Wt (i.e. the weight percentage of the total weight of chitosan and gelatin in the solvent). First, chitosan-gelatin composite scaffolds were built under different electrospinning conditions. To evaluate the morphological structure and select the optimal electrospinning conditions, some scaffolds were made using scanning electron microscopy (SEM). Then SWNTs-COOH nanoparticles with 1 different weight concentrations of 902, 9092, and 77Wt (the ratio of the weight percentage concentration of the final solution, i.e. 797Wt) were added to the CS-GEL solution and subjected to ultrasonic conditions. Finally, the solutions obtained under optimal conditions were transformed into nanofiber composite scaffolds through the electrospinning method. The morphology of CS-GEL electrospun scaffolds containing different percentages of SWNTs-COOH nanoparticles was evaluated by field emission scanning electron microscopy (FESEM) to examine scaffold morphology, Fourier transform infrared spectroscopy (FTIR) was evaluated to confirm the presence of any materials, examine a possible interaction between them, identify intermolecular interaction between electrospun nanofiber scaffolding and material miscibility, Differential Scanning Calorimeter (DSC) was used to examine the thermal behavior of scaffold components and material miscibility, and Tensile test was performed to evaluate their mechanical strength. After crosslinking with glutaraldehyde (GTA) vapor, Electrospun scaffolds were placed under water contact angle (WCA) tests to evaluate hydrophilicity, degradation test to determine degradation process, cell viability (MTT) to evaluate cell biocompatibility and non-toxicity of scaffolds and cell cultures to evaluate the adhesion, growth and penetration of cells into the scaffold. Optimization of electrospinning solutions showed that when the voltage ratio applied to the electrospinning distance increases, the average diameter of CS-GEL nanofibers decreases to 222072 ± 728011 nm, and the diameter uniformity of nanofibers increases. Moreover, the analysis of FESEM images showed that the 344 average diameter of CS-GEL nanofibers decreases by adding COOH-SWNTs nanoparticles to the scaffold content and increases their percentage to 77 by weight of 228089 ± 72092 nm. All scaffolds had porosity higher than 697 in their first layer. The FTIR and DSC analysis showed the probable increase in bond formation or stronger bonds, as well as an increase in the miscibility of the material by adding SWNTs-COOH nanoparticles to the scaffold content. By adding and increasing the weight percentage of SWNTs-COOH to nanofiber scaffolds, the mechanical properties were improved so that the tensile strength increased almost 8 times in the sample containing the weight/volume concentration of 77. WCA image analysis also showed that the hydrophilicity of the samples has increased by adding SWNTs-COOH nanoparticles to the scaffold and an increase in its percentage. Based on the degradation rate of CS-GEL cross-linked scaffolds with different durations under GTA Vapor in buffersaline phosphate solution, the optimal cross-linking time of the scaffold was 2 hours. During the biodegradability period (99 days), the weight loss percentage of the scaffold was 28022 ± 90987, which was reduced to 12 ± 90627 by adding SWNTs-COOH nanoparticles and increasing its weight/volume percentage of concentration to 77. Cell compatibility testing also showed that the produced cell scaffolds were compatible and it was found that chondrocyte cells on nanofiber scaffolds had good adhesion and growth. Therefore, CS-GEL composite scaffolds containing the weight/volume percentage concentration of 77 of SWNTs-COOH nanoparticles can be a good option for cartilage tissue engineering due to their desirable properties