The changes of urban land use/cover create air temperatures differences in various places of a city. Recognition of these differences and their effects on urban landscape water requirement is necessary for urban planners under condition of current water deficit in arid area of Isfahan to select the efficient management strategies. In this research, the air temperature and relative humidity differences at the canopy level in five intra-urban sites and one site in suburban during 42-months (October 2010-April 2014) were considered and analyzed. Five sites were different in terms of land use/cover. The highest monthly maximum, average and minimum temperature difference between the sites over the study period were 7.9, 3.8 and 2.2°C respectively which occurred in warm months. The highest difference in the hourly temperature was 12.1°C, measured at summer nights, between a botanic garden and the suburban site. The highest monthly maximum, average and minimum relative humidity differences between the stations were 25, 44 and 8%, respectively. The results showed that land use/cover affects the time that daily minimum and maximum temperature occurs. The highest mean time difference was 1 hour and 18 min, and occurred between the commercial-residential site and the suburban site in summer. Regression models developed to predict the temperature in intra urban sites based on reference suburban site temperature (R 2 = 0.84-0.99, RMSE= 0.6-1.4). Microclimate zoning of Isfahan city was done in GIS using digital land use maps (including green space, garden, agricultural land, building, street, passages, water body, population density and bare land) and based on monthly average temperature in intra urban sites, for the first and second six-months of the year separately. The result of evaluation was acceptable (R 2 = 0.89-0.92). The results of zoning for four different scenarios (A. the existence of water in river and the farms under cultivation, B. the river is dry and the farms are under cultivation, C. the existence of water in river and the farms are not cultivated, D. the river is dry and the farms are not cultivated) showed that the creation of green spaces can lead to a significant improvement in city microclimate condition. Whiles the existence of water in river (without permeation into the aquifer and creeks) improves the microclimate condition just in a radius of about 500 m from flowing water. The results showed that the microclimate zoning is a beneficial tool to consider the effects of the changes of land use/cover on microclimate condition in various places of city. The effect of land use/cover on urban landscape water requirement was investigated by calculating microclimate coefficients in intra urban sites for each month of the year. The results showed that not only the variation in place but also the variation of month can change the microclimate coefficient. Supposing a constant microclimate coefficient over the year lead to error in estimating the landscape water need. The use of a constant microclimate coefficient led to an underestimate of about 8% in the annual water requirement in the botanic garden. The water gross requirement in two different green landscape (the botanic garden and a forest park) were estimated by WUCOLS, LIMP methods and compared with applied water. Based on the actual irrigation volume and plant health and aesthetic appearance at each site, the WUCOLS method was considered to potentially underestimate plant water requirements in warm seasons. This study revealed the necessity of paying attention to the microclimate in management of landscape irrigation in Isfahan