Scarcity of freshwater resources increases the importance of seawater and brackish water desalination processes. However, large amount of specific energy requirement, and high operational costs, presents a big challenge in adoptingdesalination technologies. Due to high expenses of energy, desalination of saline waters by low-cost methods is important. This study investigated the Caspian Sea water and water well of Dark zone in Isfahan. To investigate the ability of two adsorbents (zeolite and grapheme oxide) to remove salinity ions from aqueous solutions. a fixed-bed zeolite column with graphene oxide (GO) layer was used to remove Na + , K + , Ca 2+ , Mg 2+ and Cl – from 50 cc of saline water. Also Hexadecyltrimethylamine (HDTMA) was used to modify natural zeolites. The results showed that among the adsorbents for Dark well water, 30mg graphene oxide with 13gr zeolite had the highest adsorption rate (23.84 percent of salinity reduction) and for Caspian sea water, 13gr zeolite modified by surfactants had the highest adsorption rate (23.43 percent of salinity reduction). Also the removal of cations and anion followed the sequence: K + Ca 2+ Mg 2+ Cl – Na + . Keywords: graphene oxide, desalination, fixed-bed