Apple, Malus Domestica Borkh., being produced 70 million tones annually is regarded as the most important fruit in the temperate zone. Iran, with land race apple and 2.7 million tones annual production is a principal centre of apple production in the Middle east. During post harvest operations the product is lost by almost 20 percent. ‘ Golab Isfahan’ apple is an important land race in Iran with unique flavour. Time period of supplying the apple to the market is very short and still demands exist in other seasons than time. However, its storage for long time is very difficult due to soft texture, high free water and abundant sugar content. Harvesting the fruit on physiological maturity stage that increases storage life is a main character influencing postharvest life. The aim of this study was finding the best time of harvest ‘ Golab Isfahan’ apple to increase storage life and to decrease post harvest lost of product. The fruits were picked in four maturity stages, 63, 71, 80 and 90 days after full bloom stage, from two Malling Merton 111 vegetative and seedling stocks and then stored in six different time periods of 0, 20, 40, 60, 80 and 100 days at 2°C and relative moisture of 85%. The experiment was done based on completely randomized design as split-split plot in time with 3 replications. Fruits were taken out from storage every 20 days and some physico-chemical properties of them being measured. Results showed that harvest stage significantly influenced all measured parameters (p 0.001). Increase of fruit maturity showed a decrease in weight loss percentage, fluorescence chlorophyll, chlorophyll content, flesh firmness, titrable acidity and tissue starch. However, soluble solid content, fruit water percentage and juice pH were increased. At the end of storage, least weight loss percentage was observed in third harvest of fruits (80 days after full bloom) and most weight loss was observed in first harvest.The most of fluorescence chlorophyll and chlorophyll content were found in fruits first harvest and least of these indexes were observed in fourth harvest of fruits. Best texture firmness was also measured in those third harvested however most and least of firmness was found in first and fourth harvet respectively. Highest soluble solid content percentage was again found in fruits harvested at 80 days after full bloom and least of SSC was found in fruits of 90 days after full bloom. Highest of titrable acidity was observed in fruits of third harvest and least of it was again found in last harvest. In general, changes in the fruit physico-chemical parameters during storage period indicated that third harvested preserved their quality of fruits significantly better than fruits of other harvests and showed the uppermost market acceptability at the end of the storage. Thus, the optimum harvest to increase storage life for ‘