Plane is one of important tree used in urban landscapes of Iran. In most urban lands, plane trees faced with different nutritional issues including deficiency and toxicity of mineral nutrients. Among problems, can to point out chlorosis. On the other hand, one of the most limiting factor of landscapes development is securement of water for plants. Deficit irrigation is a desirable method for saving water use in water deficit conditions and ultimately reducing necessary cost of water securement to landscape plants. Also may inoculation of plants’ root with mycorrhizal fungus can to used as method to reduce water need of plants. The current investigation was conducted as two separated experiments to evaluate the effect of Inoculation of plants with mycorrhizal fungus on response of plane trees. The first experiment was undertaken to determine the effect of inoculation with mycorrhizal fungus on response of plane saplings to different applicable water levels (securement the 50 and 100% of water need). In the second experiment, effect of mycorrhizal fungus on uptake of nutrients and severity of cholorosis in mature plane trees, was evaluated. First experiment in frame of completely randomized design as orthogonal with 6 replicates and the second one in two consecutive years as completely randomised block design with 6 replications, were carried out. The results of the first experiment showed that with exception of irrigation level, inocultion of plants with mycorrhizal fungus significantly increased majority of growth parameters viz, leaf area, chlorophyll and wet and dry weights of plane saplings. Also, the content of relative water was increased with application of mycorrhizal fungus. On the other hand, proline concentration in plane sapling leaves in deficit irrigation conditions was increased. Concentration of phosphorus in inoculated plants as compared to non-inoculated plants significantly increased too. In the second experiment, inoculation of plane trees with mycorrhizal fungus resulted singnificant increase in most growth and physiological parameters such as growth of current season shoots, soluble sugar, chlorophyll, photosythetic indices and stomatal pattern e.g. diameter and number of open stomata and also increased uptake of some nutrients such as nitrogen and zinc as compared to control. Wherease leaf chlolorosis in inoculated plants with fungus significantly reduced in comparison with control. However, mycorrhizal inoculation in most cases did not had significant difference to the rest treatments, collectively, the obtained resutls of this study revealed that inocultion of plants with mycorrhizal fungus, improved plants growth under stress conditions through its positive influence on nutrient uptake, chlorophyll content and subsequently increase in photosynthesis. It is recommended that in dry regions and water shortage conditions, the deficit irrigation method together with mycorrhizal fungus inoculation strategy will be used to reserve using water. Keyword: Landscape, Irrigation level, Plane tree, Mycorrhizal, Chlorophyll, Chlorosis, Nutrient