The use of organic material due to less side effect than chemical materials increased in agriculture and manufacturing product. Wood vinegar a substance derived from cooling carbon black fire can be used instead of chemical material in the agriculture industry as an organic compound. In order to study, effectiveness of pine wood vinegar on physiological and photosynthesis trait of cucumber and basil two experiment were conducted based on a completely randomized design with 6 treatments including 0, 1250, 2000, 2500, 3333 and 5000 mg/l of wood vinegar with 4 replications. The first experiment was designed in the laboratory in order to study wood vinegar priming on cucumber and basil seeds and the second experiment was in a greenhouse of Isfahan University of technology. The highest flower number and yield in cucumber was observed in 2500 mg/l wood vinegar. Photosynthesis in cucumber and basil increased in 2000 and 3333 mg/l, respectively. The lowest trairation in cucumber was in 2000 mg/l wood vinegar. In general, pine wood vinegar at 2000 mg/l and 2500 mg/l increased yield and quality of cucumber and basil, respectively. Wood vinegar increased germination percentage in the cucumber seeds treated with 1250 mg/l and in basil seeds treated with 2500 mg/l and speed up the germination process of both plants at 1250 mg/l. The highest root length, volume and surface as of cucumber plants were observed at 1250 mg/l, but for basil the maximum volume and length of root observed in 3333 mg/l and the highest root surface in 5000 mg/l. The concentration of nitrogen in the treatment of 3333 mg/l in both plants and the potassium concentration in the treatment of 5000 mg/l, calcium and iron in the treatment of 1250 mg/l in basil was at the highest level compared to other treatments. The highest amount of chlorophyll and photosynthesis were observed at treatments 2000 mg/l and 3333 mg/l for cucumber and basil, respectively. The treatment of 2500 mg/l in cucumber showed the highest flower and fruit yield, but the best and highest quality of fruit treated with 2000 mg/l was produced. Application of pine wood vinegar at treatments 2000 mg/l and 2500 mg/l, respectively for cucumber and basil created the highest efficiency and quality. Keywords : yield, nutrient concentration, photosynthesis, flowering, fruit