Button mushroom ( Agaricus bisporus ) from Agaricaceae family is the most common mushroom are produced in the world as well as Iran. Mushroom, like any other plant organism has its own dietary needs. Its food requirements will be provided through the compost. Macro and micro elements must exist to the extent required for suitable yield. Therefore In this study, the effect of adding manganese and iron to the bedding compost of on yield and quality of the mushroom were investigated. This study were done in Kabir unit of Yekta Mushroom Company is located near Jarghoye region in 1392 (2013). Two separate experiments were conducted in a randomized complete block design. In the first experiment to determine the appropriate concentration of manganese and iron, seven treatments were used. In addition to the control (no addition of iron and manganese), the 3 concentrations of 100, 200 and 300 mg of Mn per kg dry substrate, and three concentrations of 250, 500 and 750 mg of Fe per kg dry substrate was used. The second experiment was planned based on the results of the first experiment. In this experiment 4 treatments containing 300 Mn, 500 Fe, 300 mg Mn + 500 mg Fe per kg of dry substrate and control were used. For both expriments necessary amount of manganese sulfate and ferric sulfate for every 4 of blocks of compost (an experimental unit) was calculated and disored in a liter of distilled water and sprayed on the surface of the compost penetrate to a depth of 5 cm. Harvesting was done at the 3 Flash. In addition to measuring the overall yield of each exprimental unit, test samples of 400 g (250 g in the second experiment), was selected. Fresh and Dry weight of the cap and stipe, diameter and length of the cap stipe was measured. In the second flash in addition to the previous mentioned samples, a sample for measuring firmness, soluble solids, extract pH, organic acids, L-value, weight loss during storage, the marketability, concentration of manganese and iron in tissues of button mushroom was taken.The results of the first experiment showed that the highest yield, diameter and length of stipe, respectively was observed in, treatments of 300 mg Mn and 500 mg of iron per kg dry substrate where significantly increased 17.7% and 16.5% when compared to control. The maximum diameter of the cap was obtained by treatments of 500 and 300 mg per kg of iron and manganese, respectively. Treatments showed no significant effect on percentage of fresh weight of cap stipe, dry matter of cap stipe, percent of weight loss organic acids, marketability, firmness, total soluble solids, pH, Whiteness. Adding iron to the planting bed reduced the amount of iron in the tissues compared with the control. The second study showed that the two treatments of 300 mg of Mn and 500 mg Fe per kg of dry substrate increased the yield by 15.65% and 13.4% respectively. But the combination of both Mn and Fe hadn't significant effect on the yield compared to control. The cap diameter, stipe diameter and length were increased significantly by 300 mg Mn and 500 mg Fe per kg a substrate comparet to control. Finally, it can be concluded that 300 mg Mn and 500 mg Fe per kg of dry substrate can increase the yield. Key words: Button mushroom, Fe, Mn, Quality, Yield.