Drought is one of the most important abiotic stresses affecting plant growth and development. Nutrient application i potent to enhance resistance to biotic and abiotic stresses. This experiment was conducted to study the effect of two types of zinc fertilizer on drought tolerance of Grapewine cultivars ‘Askari’, ‘Yaghuti’ and ‘Rishbaba’. one-year-old potted cuttings of Grapewine were subjected to two irrigation regimes (50% and 100% of the FC) and then they were treated with the 5gl -1 of zinc sulfate or zinc anino chelate twice with 1 week intervals. The results showed that cultivar ‘Askari’ through better control of ion leakage during stress, decreased node number, increasing in root volume and weight, chlorophyll fluorescence (Fv/Fm), chlorophyll and carotenoid contents and chlorophyll index (spad) appear to be most drought- resistant than other studied cultivars. ‘Yaghuti’ had the highest shoot growth and the lowest root growth and was relatively drought susceptible. Spraying of zinc sulfate under 100% FC irrigation regime significantly improved root and shoot growth. Zinc sulfate application did not affect leaf proline content, but it enhanced node number and root volume of ‘Yaghuti’ under drought-stressed conditions, leading to it's susceptibility to drought. Zinc amino chelate spraying reduced root length and Fv/Fm and increased node number and ascorbate peroxidase activity. In cultivar ‘Yaghuti’, shoot growth increased and root growth decreased under water stress treatment with zinc amino chelate application compared with the control treatments. Therefore, it could be concluded that ‘Askari’ cultivar had high drought tolerance and ‘Yaghuti’ had the most sensetivity to drought condition among these cultivars and also various treatment of Zn source could not enhance drought tolerance in senseitive cultivar. Keywords : Irrigation, Grapewine, Water stress, Spraying