Alstroemeria, also known as Peruvian lily, is an herbaceous, rhizome, and monocotyledonous plant from the Alstroemeriaceae family. It is highly important in global markets due to its variety of flower color and shape, ease of cultivation and long flowering life. In traditional Alstroemeria proliferation, which is carried out by rhizome division, the proliferation rate is low and the risk of viral disease spreading is high. In light of high heterozygous characteristics of this plant and lack of need for seeds, the use of micropropagation techniques for mass and rapid production of Alstroemeria seems essential. In order to evaluate the regenerative ability of different parts of this plant under in vitro condition, immature inflorescences, rhizomes and terminal meristems were used for direct organogenesis and node explants for callus production. In regeneration of immature inflorescences using preliminary experiments, the appropriate explant size was determined. In the stages of plant regeneration from immature inflorescences explant, the whole plant was produced from immature flowering explants using different concentrations of BAP and KIN with 1 mg/l NAA in factorial experiment (4×4) and a completely randomized design. The highest branching induction (4.1) and the maximum number of roots (3.87) were obtained at a concentration of 3 mg/l BAP and KIN. At a concentration of 2 mg/l BAP and KIN, the largest branch diameter (6.93 mm) was obtained. The terminal meristem continued to grow at auxin/cytokinin levels for a long time without generation of roots or lateral branches. Rhizome explants failed to regenerate despite high fungal infection. Node explants failed to produce callus using different combinations of auxin and cytokinin hormones. The plants that generated roots and stems were transferred to cocopeat and perlite culture medium with a 1:1 mixture for adaptation and the entire plant was produced in pots. Keywords Alstroemeria, tissue culture, immature inflorescences, rhizome, micropropagation