Microprpagation of Lemon Verbena ( Lippia citriodora ) and Application of Methyl Jasmonate and Salicylic Acid on Increasing of Secondary Metabolism Akram Auobi Akram_auoby @yahoo.com Date of submission:May20, 2012 Department of Horticultural Science Isfahan University of Technology, Isfahan 84156-83111, Iran Degree: M.Sc Language: Farsi C. Ghobadi, Ph. D., Assist. Professor (Supervisor), cyrus@cc.iut.ac.ir Plants have been used as medicines throughout history and are main sources for medicine and pharmacy in most of the world. According to statistics reported by World Health Organization (WHO), the plants are one of the most important medicine sources for the man. produced plant Based Drugs, are types of Secondary Metabolites. Secondary metabolites are chemicals produced by plants under unnatural conditions. Lippia citrodora is a perennial plant and belongs to verbenaceae family. Sedative and Antibacteria effects are properties of this herb. This plant has antioxidant, antimicrobial and antitumore properties due to phenolic compounds (flavonoids and phenolic acids) and terpenoids. The leaves are very aromatic and has frequently applied as a tea. This plant has several essential oil and Its main components include cineol, Neral and Lymonen. Based on the medicinal importance of Lippia citrodora , the study was performed to propagation by tissue culture and increase of secondary metabolites. This study Was conducted in two experiments. In first experiment, tissue culture of Lippia citrodora with two type of explants (node and shoot tip) and 16 hormonal combination (consist of 0, 0.1 0.3 and 0.5 NAA and 0, 1, 2 and 4 BAP) with four replications was studied. The shoot number, shoot length, node and leaf number, internode length, callus and root formation were measured. The highest growth rate was obsereved on node explant and medium with 0.3 NAA without BAP. The results showed that hormonal combination of NAA without BAP had a positive effect on Rooting and increase of NAA to 0.3 mg enhanced Rooting and concentrations up to this, concentration caused rooting failure. Also observed that in node explant and hormonal combination of 0.1 mg l NAA, 1 mg BAP were the most effective in shoot product. the maximum length of shoots was obsereved in treatment containing 0.3 mg NAA, 0 mg BAP. In second experiment, effect of methyl jasmonate and salicylic acid was studied to increase of Secondary Metabolites in Lippia citrodora with eight treatments (0,12.5, 25 and 50 mg/l methyl jasmonate and 100, 200 and 400 mg/l salicylic acid) in three replications was studied. The Clevenger and Gas chromatography–mass (GC-Mass) were used for essence extraction and detect of essence compounds respectively. The results showed that although hormonal treatments increased some of compounds, various concentrations had different effect. The 1,8-cineole was increased in 100mg/l salicylic acid and 12.5 mg/l methyl jasmonate. The naral and genarial compounds were increase with 400mg/l salicylic acid and 12.5 mg/l methyl jasmonate respectively. Key words : Lippia citrodora , Microprpagation, Methyl jasmonate, Salicylic acid and Secondary Metabolites.