Paclobutrazol (PBZ) is a member of the triazole plant growth inhibitor group that is responsible for inducing tolerance to number of biotic and abiotic stresses. An experiment was, therefore, conducted to test whether PBZ application at various concentrations (0, 25, 50 and 75 mg L -1 ) through foliar spray or soil drench would protect pomegranate ( Punica granatum ) seedlings cv Rabab, subjected to freezing stress.Three weeks after the PBZ application seedlings exposed to freezing in a growth chamber at -3 ? c for 7 hours and then transfered to the greenhouse with average temperature 25 ? c. seedling were assessed 48 hours after the end of fressing stress to determine the extent of freezing injury. the layout was 2×4 factorial experiment in a complete randomized design with four replication. PBZ improved growth rate of pomegranate seedling subjected to freezing stress and increased relative leaf chlorophyll content (RLCC) chlorophyll fluorescence ratio (Fv/Fm), net photosyntisis and stomatal conductivity of leaf, relative water content, soluble carbohydrate content and enzyme activity of peroxidase and ascorbat peroxidase compared with the control at the end of experiment. PBZ ameliorated the injury caused by freezing stress by inhibiting increases in proline and leaf electrolyte leakage, which suggested that PBZ ameliorated the negative effect of freezing stress. In the most evaluated factors no significant difference among two foliar spray and soil drenchtreatment observed. The best protection to be obtained from seedlings treated with PBZ at 50 or 75 mg L -1 . The results indicated that PBZ was considerably effective to reduce the damage of low temperature-induced stress in pomegranate seedling. Key Words: paclobutrazol, pomegranat, cold hardiness ، peroxidase enzyme