Some common environmental stresses such as drought, salinity and cold adversely affect plants to perform their complete genetic potential and have constraint effects on crop growth and yield. Plants may tolerate and adapt to these stressors by mechanisms at physiological, biochemical and molecular levels. Molecular studies have shown that many genes can be induced by stressors through stress signaling and gene expression. The expressed genes, based on their characteristics and the products can be categorized into two groups, including those encode functional proteins and the genes that their products are regarded as the most important components for deciphering signal transduction of stresses and expression controls of stress tolerant genes. Transcription factors are critical regulators of gene expression system that play central roles in regulating downstream genes contributing to stress tolerance and the regulatory role of DREB transcription factors have been extensively studied. DREB transcription factors are one of the four independent regulation systems in plants which involves in abiotic stress signaling pathway and activation of stress responsive genes. The objectives of present study were to identify, isolate and investigate the VvDREB and orthologous SbDERB2A genes from grapevine ( Vitis vinifera L.’ Askari’) and the constitutive expression of VvDREB gene under salt stress conditions in Tobacco model plants. Grapevine vitroplants were subjected to salt stress (150 mM NaCl) for 24 hours and total RNA was extracted. Specific primers were designed based on grapevine’s drought EST and SbDREB2A gene homologue in halophilic plant Salicornia brachiata . VvDREB and VvsDREB ( DREB like 2C) genes were isolated from grapevine ( Vitis vinifera L.’ Askari’). Bioinformatics results indicated that VvDREB gene has 85% similarity with DREB2 genes from soybean family and VvsDREB gene has 80% similarity with SbDREB2A gene . SbDREB2A and VvsDREB proteins motif studies in ELM database showed that there are no five motifs (BRCT, MAPK, PKB, NES, NLS) in VvsDREB protein. A cassette consisting of grapevine VvDREB cDNA driven by CMV35S promoter was cloned into pBI121 binary vector and transferred into Agrobacterium tumefaciens strain LBA4404. Leaf disks from vitroplants were co-cultivated with A . tumefaciens and transformants under salt stress and water deficit conditions were selected. The presence and expression of the gene in transformants were confirmed by molecular methods, PCR and RT-PCR techniques, respectively. Increasing tolerance to salinity and drought were observed in transformed plants compared with the control plants. Overall, it could be concluded that the VvDREB gene belongs to DREB2 family in plants. Keywords : Vitis vinifera L., DREB, Salt stress, Water deficit, Salicornia brachia