Cooperation of both environmental elicitors and regulatory cis-acting elements in gene promoter, plays significant role in expression of genes under variable environmental conditions. Rd29A promoter is a stress-inducible promoter, which minimizes negative effects on plant growth and development, while applied instead of constitutive promoters. In this study, in order to isolation and characterization of rd29A promoter of Arabidopsis , total DNA was isolated from this plant. Specific primers based on the upstream sequence of rd29A gene were designed by oligo analyser, and two regions of rd29A promoter with 899 bp and 1260 bp lengths were isolated, followed by cloning into pTZ57R vector. Regulatory elements existing on these two promoters were identified in PlantCARE database. Alignment of studied sequences showed 100% similarity with previously reported rd29A promoter in NCBI database. In order to evaluate the activity of rd29A promoter in petunia plant, six constructs including gus , DREB1B , and nptII genes were prepared under inducible rd29A promoters of two distinct 899 bp and 1260 bp lengths in pBI121 binary vector, and pBI121:rd29A-1260: nptII , pBI121:rd29A-899: nptII and also pBI121 as control, were transformed to petunia via strain GV3101 Agrobacterium tumefaciens bacteria. Transgenic plants grown in cultures containing kanamycin 100 mg/ml, were exposed to salt stress (200 mM NaCl) and resistant plants were selected. Presence and activity of rd29A promoter in transgenic plants were verified by molecular tests, PCR and RT-PCR. Induction of rd29A promoter in transgenic plants resulted in higher resistance to kanamycin under salinity, in comparison to non-transgenic plants. Results of molecular and physiological surveys of rd29A promoter demonstrated that this promoter, unlike constitutive nos promoter, is induced just under stressful conditions. Moreover, physiological results from transformation of pBI121:rd29A: nptII constructs verified the consequences of bioinformatics analysis which depicted higher expression of nptII gene under salt stress in transgenic plants bearing pBI121:rd29A-1260bp: nptII construct. Considering the fact that, permanently expression of stress-resistance genes under constitutive promoters usually obstacles normal growth of transgenic plants, therefore, inducible rd29A promoter can be a proper substitution for generating stress-resistant plants. Keywords : inducible rd29A promoter, nos promoter, regulatory elements, PlantCARE, gus , DREB1B , nptII