Saffron ( Crocus sativus ) from Iridaceae family is known as the most expensive spice in the world. Saffron has six key stages in its life cycle. Among those, the flowering stage of saffron is importance. Many genes control the flowering process in this plant, which the AP1 gene is referred as flowering process markers. Various compounds are produced in the saffron stigma, which carotenoids are the most important and significant combination of saffron straw among them and they are responsible for the color of this spice. The final stage of the production of carotenoids, which includes the glycosylated of the compounds resulting from the loss of Zeaxanthin, is carried out by the Glycosyl transferase 2 enzyme coded by the UGTCs2 gene. There is family MYB transcription factors of upstream of both AP1 and UGT genes. The studies show that the expression of this gene is affected by plant hormones.Therefore, the aim of this study is investigating the effect of different hormones on the expression of MYB gene as well as AP1 and UGT genes. For this purpose, saffron corms were treated with Gibberellic acid (100 and 200 ppm), Salicylic acid (50 and 100 ppm)- Kinetin (10 and 20 ppm), Gibberellic acid (200 ppm)- Kinetin (20 ppm), Salicylic acid (ppm 50) - Kinetin (20 ppm), Gibberellic acid (200 ppm) - Salicylic acid (100 ppm) - Kinetin ??(20 ppm) and Auxin (100 ?g / Corm) - Gibberellic acid (100 ?g / Corm) hormonal treatments. After planting and before sampling, hormonal treatments were again applied as irrigation, and the leaf tissue sampled at 12 and 24 hours after treatments. To do real-time PCR, the specific primers were designed with using oligo7 software and the changes of genes expression were evaluated as hormonal treatments. In this study, there was no adequate amplification using primers design based on MYB gene, despite the studying of different pair primers. The real-time PCR results showed that Kinetin in the concentrations of 10 and 20 ppm in both sampling times, Salicylic acid at 50 and 100 ppm concentrations in both sampling times, Gibberellin in the concentration of 200 ppm in 12 hours after hormonal treatment, the hormone combination of Gibberellin 200 ppm -Salicylic acid 100 ppm -Kinetin 20 ppm in both sampling time and Salicylic acid 50 ppm- Kinetin 10 ppm in both sampling time significantly increased the expression of AP1 gene, but Gibberellin in the concentration of 200 ppm in both sampling times and in the concentration of 100 ppm at 12 hours and the hormonal combination of Auxin 100 ?g / Corm 100 - Gibberellin 100 ?g / Corm 100 g in both sampling times and Gibberellin 20 ppm- Kinetin 200 ppm in both sampling times significantly decreased in AP1 gene expression. In the case of UGT gene, Kinetin hormones in the concentrations of 10 and 20 ppm in both sampling times, Salicylic acid in the concentration of 100 ppm in both sampling times and in 50 ppm concentration at 24 h after hormone treatment, hormonal combinations of Gibberellin 200 ppm -Salicylic acid 100 ppm -Kinetin 20 ppm, and also Gibberellin 200 ppm-Kinetin 20 ppm resulted in the significant increase of expression, and other compounds did not significantly change the expression of this gene. According to the results, Kinetin was considered as the best hormone for inducing of both AP1 and UGT genes expression. Keywords: Saffron, Flowering, Hormone, AP1 , UGT , MYB , Real-time PCR.