Pistachio ( Pistacia vera L.) is a member of Magnoliophyta division, dioecious, deciduous, living in the arid regions and wind-pollinated in Anacardiaceae family. Traditional techniques for propagation of pistachio are inadequate, time-consuming and low efficiency, consequently, new techniques of plant propagation for increase production efficiency and decrease cost must be used. Development of biotechnology methods such as plant tissue culture improved agricultural field in recently years. Tissue culture of pistachio began in the early 1980s and until now it has been suggested various culture media for growth and establishment. In-vitro propagation of this planthas some limitations such as inaccessibility of suitable explant with low contaminations, phenol exudation followed by explants died, low ratio of establishment, proliferation and rooting and finally shoot tip necrosis. The focus of thesis achieved a protocol for propagation of this plant and considering the integrity of genetic diversity of regenerated plants compared to native plants (somaclonal variation) using SRAP Markers. To disinfection of explant used different concentrations and times of sterilizing agent. In the next experiment, to counteract obstacle of phenol exudation in media culture before established the explant in medium, preparing immature explant in active seasons of the plants, added anti-oxidant in media culture, use of different concentrations of media and various amount of plant growth regulators and selected of height explant were considered. Proliferation rate using many types of media and plant growth regulators, changed the ferrous source, selected the appropriate explant from the establishment stage, use of adenine, peptone and silver nitrate in media culture. In rooting stage, change the concentration of media culture, replacement of plant growth regulator and use of activated charcoal was evaluated. Ten pair primers of SRAP used to evaluating of somaclonal variation. The results of this study showed that the best treatment for disinfection of 1-year explant that keeps in the greenhouse was 10% home detergent (with 5% Sodium hypochlorite) in 10 min., followed by ethanol 70% in 60 seconds and disinfection of stems that collects from the field was 20% home detergent (with 5% Sodium hypochlorite) in 10 min., followed by ethanol 70% in 120 seconds. The best seeds disinfection treatments were mercuric chloride with 0.1% in 10 mins. In proliferation stage use of DKW media culture with red ferrous sequestrene together with 3 milligrams per liter benzyl aminopurine and 0.3 milligrams per liter gibberellic acid with 100 milligrams per liter vitamin C was shown 8-10 rate of proliferating. Explant that had appropriate quality and size transfer to rooting media included ½ concentration of macro and micronutrients DKW media with 20 gram per liter sucrose and 2 milligrams per liter of indole-3-butyric acid for a week and after that subcultured to ½ macro and micro elements of DKW media culture without any plant growth regulator caused the emergence of roots. The results showed that regenerated plants were not significantly different from mother plants and somaclonal variation was low. It seems that because of using nods of stem caused their similarity was high.Using tissue culture-based biotechnology techniques to propagate and proliferate plants are highly efficient and commercially profitable. Key Words : Pistachio, UCB1, Mircopropagation, Tissue Culture, Somaclonal Variation, SRAP.