The commercial production of plant is growing worldwide. Its monetary value has significantly increased over the last two decades and there is a great potential for continued further growth in both domestic and international markets. Regenerated and transformed petunia plants ( Petunia hybrida cv. Supercascaide; Petunia hybrida cv. Opera; Petunia Nictaginiflora ) for Iranian and forein cultivar have been produced by means of shoot apex and leaf disc Agrobacterium -mediated transformation method. Seeds of different cultivars were grown in MS medium for regeneration via shoot apex. Shoot apex were isolated from seven days seedlings and in order to select the best shooting media, the statistical analysis explants in a CRD with 12 shooting treatments: MS + B5 vitamins with different concentration of NAA (0, 0.2, 0.5) and BAP (0, 0.1, 0.5, 1). The statistical analysis indicated that the best for producing multiple shoot, was medium with 1mg/l BAP. Optimizing regeneration via leaf disc, explants of different cultivars were put in three modified MS media and Supercascaide leaf discs were put in 10 different medium that were different in concentration and kind of growth regulators. The results showed that the best medium for direct regeneration was MS3 medium. Of course mean comparisons between media and Petunia hybrida (Opera, Supercascaide) and Petunia Nictaginiflora indicated that the best for producing direct shoot, were Nictaginiflora species and Supercascaide cultivar in MS 3 and MS 12 , respectively. The best for producing indirect shoot was Opera in MS 3 medium. The leaf disc and shoot apex were co-cultivated with Agrobacterium strain LBA4404 harboring a plasmid pBI121 was used as the vector system for transformation. pBI121 contained ?-glucuronidase ( gus ) gene as a reporter gene and pyroline 5-carboxilate synthase ( p5cs ) driven by the cauliflower mosaic virus (CaMV35S) promoter and neomycin phosphotransferase ( npt II) gene used as a selectable marker. The leaf disc and shoot apexes were transferred to selective medium containing Kanamycin (200 mg/l) and then transferred to proliferation medium (modified MS with 1 mg/l BAP). The cultures were kept at 25±2°C and 16/8 hour photoperiod under cool-white fluorescent light at 40 µmolm -2 s -1 . Shoot regeneration occurred within 3 weeks. To confirm transformation, regenerated plants were subjected to the polymerase chain reaction (PCR). GUS and proline assay confirmed the perscence of the gus and p5cs gene in the genome of all transformants in stress and nonstress conditions