: In the first experiment, based on pedologic and geomorphic characteristic of Pb and Zn mines, Anguran mine in Zanjan province, Bama mine in Isfahan province and Sormeh mine in Fars province were selected. The soils derived from mine parent materials were studied in a detailed scale. The research sites were selected based on the obtained data. The soil and plant samples were collected from natural metal polluted soils. The concentration of Pb and Zn were measured in the collected sampled. Uptake Index was calculated both for pb and Zn. The highest amount of Zn Uptake index obtained for stipa barbata where the maximum Pb Uptake index obtained for Kochia scoparia. In the second experiment, phytoextraction of Lactuca sativa, Spinacia olereacea, Brassica oleracea, Beta vulgaris, Lolium persicum, Bromus beneckenii, Secale, Zea mays, Helianthus annuus and brassica napus were investigated in a pot experiment. The treatments were two levels of chelation agents (1.5 and 3.0 mmoles DTPA per kilogram of dry soil), two levels of sulfuric acid (0.5 and 1 mmole sulfuric acid per kilogram of dry soil) two levels of composted manure ( 7.5 and 15g of composted manure per kilogram of dry soil) and control. The experiment was conducted in a factorial arrangement based on completely randomized block design with 3 replications in greenhouse.The results showed that , DTPA, sulfuric acid and composted manure treatments increased the availibility of soil Pb and Zn. The treatments also inereased the concentration of metals in the shoot and root of studied plants but not with the same pattern. In addition Zea mays and Helianthus annuus showed the maximum potential for Zn extraction where Lactuca sativa and Lolium persicum showed the maximum removal potential of Pb from studied soil. In the third experiment, hydrated zinc sulphate (ZnSo4, 7H2O) was activated in Isfahan Miniator Neutron Source Reactor. Activated zinc (65Zn) was mixed with a virgin soil by a rate of 0 (control ), 20, 100 and 200 mg per kilogram of dry soil. The seed of Zea mays were sown in treated pots based on completely randomized block design with 4 replications. The results showed that by addition of different levels of 65Zn, root, stem and leaf dry biomass of Zea mays did not increase. The concentration of 65Zn in different part of Zea mays tissues increase as the dose of 65Zn application increased. The soil samples were collected in three stages (Initial sampling, after 4 weeks incubation and after harvesting). Sequential extraction of the second stage samples showed exchangeable Zn fraction, Zn bound to organic matter and Zn bound to Fe and Mn oxides were increased while sequential extraction of the third stage samples showed a decrease in exchangeable Zn fraction, Zn bound to organic matter and an increase in Zn bound to carbona