Zinc (Zn) deficiency is important nutritional problem in arid and semi-arid regions of the world where cereal crops are the main staple food in these regions. One effective solution in correcting Zn deficiency is using agronomic biofortification including application of manure. Manure application will improve soil physical, chemical and biological properties. However, by addition of manure toxic heavy metals such as cadmium (Cd) and arsenic (As) may also enter the soil. The presence of As especially in poultry manure is of concern. On the other hand, long term application of animal manures may cause heavy metal accumulation in soil and groundwater. This study was conducted to evaluate the effect of applying poultry and cow manures and zinc soulphate (ZnSO 4 ) on zinc, cadmium and arsenic concentrations and uptake by two wheat genotypes. The experiment was conducted in a factorial complete random design with three factors including two wheat genotypes ( Triticumaestivum L.cv . Back cross roshan and Triticumaestivum L.cv. Kavir), two animal manures (poultry and cow) at two levels 10 and 20 ton per hectare and zinc soulphate (ZnSO 4 ) at 20 and 40 kilogram per hectare and using soils (Roodasht (Typic haplo cambids) and Lavark (Typic haplargids). Seven months after wheat cultivation root, shoot and grain yield and zinc concentration and uptake were measured. Results showed that addition of the manures soil increased DTPA extractable Zn in both soils. In addition, the soil organic matter content was also significantly increased Furthermore, the addition of the manures increased zinc concentrations in wheat roots and shoots, and the greatest zinc concentration in grain was observed i zinc soulphate (ZnSO 4 ) treatment. Grain arsenic concentration increased in both wheat genotypes after using poultry manure treatment. Also, the greatest cadmium concentrations in wheat grain were related to applying cow manure treatment compared with other treatments. However, grain wheat protein content in the manures treatments was higher than zinc soulphate (ZnSO 4 ) treatments. In addition, transfer factor of zinc (TF) of soil to root and grain wheat in the manures treatments were more effective than other treatments. The wheat root, shoot and grain yield were significantly effected in different treatments. It was observed that Kavir genotype had a significantly greater root, shoot and grain yields compared to ROOSHAN. Also, zinc concentration in shoot and grain in Back cross Roshan was higher than Kavir. It was showed that grain protein content in Kavir genotypes was greater than in Back cross Roshan. Also, there was a positive correlation between Zn concentration of grains and grain protein content and soil nitrogen content. Kay words: Agronomic biofortification, Cow manure, Poultry manure, Arsenic, Cadmium, Zn efficiency, Wheat.